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Title: Designing a synthetic tumour suppressor protein
Authors: SEE HAI YUN
Keywords: aptamer, scaffold proteins, eIF4E,
Issue Date: 5-Jun-2009
Citation: SEE HAI YUN (2009-06-05). Designing a synthetic tumour suppressor protein. ScholarBank@NUS Repository.
Abstract: One of the relatively new techniques for target validation is the use of protein aptamers that bind to the target protein. Protein aptamers are small, synthetic, recognition molecules that consist of bioactive binding peptides that are constrained within a stable, compact and biologically inert scaffold protein. Binding protein aptamers can be selected from an aptamer library which consists of randomized peptides individually inserted into a particular scaffold, using yeast-two hybrid or phage display techniques. Alternatively, protein aptamers can be constructed by rational design which involves the insertion of known target protein binding peptide sequences into a scaffold, followed by optimization of the aptamer based on structural analysis, for improved binding. The aim of this project is to develop bioactive protein aptamers that bind to a selected target protein: eIF4E, a rate limiting translation initiation factor that is essential for the initiation of cap-dependent translation.
Appears in Collections:Ph.D Theses (Open)

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