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Title: Structure determination of Midkine-a
Authors: MENG DAN
Keywords: Structure determination, Midkine-a
Issue Date: 6-Jul-2009
Citation: MENG DAN (2009-07-06). Structure determination of Midkine-a. ScholarBank@NUS Repository.
Abstract: In this study, the solution structure of Mdka is solved based on the multi-dimensional NMR spectroscopy using a novel assignment method developed by our group, which shows that this method works as well for small proteins as larger ones. Mdka contains two domains, and each of them consists of three anti-parallel beta-sheets, resembling that of human Midkine. The N domain backbone architectures of Mdka and human Midkine are essentially the same, but C domains have major differences, which could be the reason for the differential functions of the two proteins. Our results provide new insights into the functional mechanism of the Midkine family; and directions for future structural study of this protein include: 1) comparing the structures of Mdka and Mdkb to understand why they have different functions; and 2) mapping the heparin binding site of Mdka to identify the specific residues important for the function of the protein.
Appears in Collections:Master's Theses (Open)

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