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Title: Phosphoregulation of the actin cytoskeleton during endocytosis in the yeast saccharomyces cerevisiae
Keywords: actin cytoskeleton, endocytosis, phosphoregulation, saccharomyces cerevisiae, Arp2p, Prk1p
Issue Date: 21-May-2008
Citation: JIN MINGJI (2008-05-21). Phosphoregulation of the actin cytoskeleton during endocytosis in the yeast saccharomyces cerevisiae. ScholarBank@NUS Repository.
Abstract: Abstract Endocytosis requires appropriately controlled actin assembly and disassembly at specific steps. Actin polymerization is initiated by the Arp2/3p complex. In budding yeast, the Arp2/3p complex is activated by several nucleation promoting factors including Pan1p. Phosphorylation of Pan1p and other components of the coat complex by the kinase Prk1p lead to termination of actin polymerization and disassembly of the coat complex. A homologous kinase, Ark1p, has also been implicated in this regulatory process. In this study, the distinct roles of Prk1p and Ark1p were investigated. We found that the non-kinase domains determined the functional specificity of the two kinases. A short region located adjacent to the kinase domain unique to Prk1p was found to be required for the kinase to interact with Arp2p. Further studies demonstrated that the Prk1p-Arp2p interaction is essential for down-regulation of Pan1p. These findings suggest an auto-inhibitory mechanism that coordinates actin assembly and disassembly during endocytosis.
Appears in Collections:Ph.D Theses (Open)

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