Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/28239
DC FieldValue
dc.titleIn vitro and in vivo characterisation of recombinant lactobacilli expressing house dust mite allergen
dc.contributor.authorLIEW LEE MEI
dc.date.accessioned2011-11-08T18:02:30Z
dc.date.available2011-11-08T18:02:30Z
dc.date.issued2009-05-04
dc.identifier.citationLIEW LEE MEI (2009-05-04). In vitro and in vivo characterisation of recombinant lactobacilli expressing house dust mite allergen. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28239
dc.description.abstractThe allergen specific immunotherapy has been the treatment of choice against type I allergy. A new generation of oral vaccines is being developed using specific strains of lactobacilli. In our study, two Lactobacillus strains were genetically engineered to express a major house dust mite allergen by using an inducible pSIP expression system. The in vitro immunomodulatory effects of recombinant lactobacilli particularly on dendritic cells were characterized. Furthermore, their immunogenicity and prophylactic potential were evaluated in murine models. These findings on recombinant Lactobacillus strains will make valuable contributions in the development of safe and more efficacious Lactobacillus-based oral vaccine for the management and treatment of mite allergy in children.
dc.language.isoen
dc.subjectLactobacillus plantarum NC8, Lactobacillus rhamnosus GG, Blomia tropicalis, allergy, immunomodulatory, recombinant
dc.typeThesis
dc.contributor.departmentPAEDIATRICS
dc.contributor.supervisorCHUA KAW YAN
dc.description.degreeMaster's
dc.description.degreeconferredMASTER OF SCIENCE
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Master's Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Lee Meis Thesis - Characterization of Recombinant Lactobacilli Expressing HDM Allergen.pdf1.97 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

257
checked on May 22, 2019

Download(s)

339
checked on May 22, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.