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Title: Feasibility of probiotic lactobacillus and yeast as oral vaccine carrier against coronaviruses
Authors: HO PHUI SAN
Keywords: coronaviruses, adhesion capabilities, oral vaccination, immunological responses, neutralizing activities, cell-mediated responses
Issue Date: 6-Dec-2006
Citation: HO PHUI SAN (2006-12-06). Feasibility of probiotic lactobacillus and yeast as oral vaccine carrier against coronaviruses. ScholarBank@NUS Repository.
Abstract: This study investigated the potential of using Lactobacillus spp. and yeast as candidate for oral vaccination against coronaviruses. The adhesion capabilities of Lactobacillus casei Shirota (LcS) and three yeast strains (Saccharomyces boulardii, Saccharomyces cerevisiae, Pichia pastoris) to murine intestinal tract were first assessed. Results demonstrated the ability of LcS and the three yeasts to adhere and replicate within murine intestine. In particular, P.pastoris was able to re-adhere better to the intestinal surfaces. Recombinant LcS and P.pastoris were engineered to constitutively express the N-terminal fragment of Transmissible Gastroenteritis coronavirus spike protein. In addition, recombinant P.pastoris encoding the receptor-binding domain of Severe Acute Respiratory Syndrome coronavirus spike protein was also generated. Oral administration of recombinant LcS or P.pastoris to Balb/c mice elicited antigen specific immunological responses. Antibodies induced showed significant neutralizing activities, though the inhibitory effects induced by recombinant LcS was apparently lower. Nevertheless, cell-mediated responses were also mediated in mice orally immunized with recombinant LcS or P.pastoris.
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