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https://doi.org/10.1016/j.cardiores.2007.05.026
Title: | The novel proangiogenic effect of hydrogen sulfide is dependent on Akt phosphorylation | Authors: | Cai, W.-J. Wang, M.-J. Jin, H.-M. Yao, T. Zhu, Y.-C. Moore, P.K. |
Keywords: | Angiogenesis Endothelial cells Migration |
Issue Date: | 2007 | Citation: | Cai, W.-J., Wang, M.-J., Jin, H.-M., Yao, T., Zhu, Y.-C., Moore, P.K. (2007). The novel proangiogenic effect of hydrogen sulfide is dependent on Akt phosphorylation. Cardiovascular Research 76 (1) : 29-40. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cardiores.2007.05.026 | Abstract: | Objective: Hydrogen sulfide (H2S) has been reported to be a gasotransmitter which regulates cardiovascular homeostasis. The present study aims to examine the hypothesis that hydrogen sulfide is able to promote angiogenesis. Methods: Angiogenesis was assessed using in vitro parameters (i.e. endothelial cell proliferation, adhesion, transwell migration assay, scratched wound healing and formation of tube-like structure) and in vivo by assessing neovascularization in mice. Phosphorylation of Akt was measured using Western blot analysis. Results: Exogenously administered NaHS (H2S donor) concentration-dependently (10-20 μmol/l) increased cell growth, migration, scratched wound healing and tube-like structure formation in cultured endothelial cells. These effects of NaHS on endothelial wound healing and tube-like structure formation were prevented by either the phosphatidylinositol 3-kinase (PI3K) inhibitor LY 294002 (5 μmol/l) or transfection of a dominant-negative mutant of Akt. NaHS increased Akt phosphorylation and this effect was also blocked by either LY 294002 or wortmannin (25 nmol/l). NaHS did not significantly alter the levels of vascular endothelial growth factor, mRNA expression of fibroblast growth factor and angiopoietin-1, or nitric oxide metabolites. NaHS treatment (10 and 50 μmol kg- 1 day- 1) significantly promoted neovascularization in vivo in mice. Conclusion: The present study reports a novel proangiogenic role of H2S which is dependent on activation of Akt. © 2007 European Society of Cardiology. | Source Title: | Cardiovascular Research | URI: | http://scholarbank.nus.edu.sg/handle/10635/27300 | ISSN: | 00086363 | DOI: | 10.1016/j.cardiores.2007.05.026 |
Appears in Collections: | Staff Publications |
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