Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jaci.2007.02.032
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dc.titleIdentification and characterization of a novel allergen from Blomia tropicalis: Blo t 21
dc.contributor.authorGao, Y.F.
dc.contributor.authorOng, T.C.
dc.contributor.authorTay, S.L.
dc.contributor.authorYap, K.H.
dc.contributor.authorChew, F.T.
dc.contributor.authorWang, D.Y.
dc.date.accessioned2011-09-19T07:41:37Z
dc.date.available2011-09-19T07:41:37Z
dc.date.issued2007
dc.identifier.citationGao, Y.F., Ong, T.C., Tay, S.L., Yap, K.H., Chew, F.T., Wang, D.Y. (2007). Identification and characterization of a novel allergen from Blomia tropicalis: Blo t 21. Journal of Allergy and Clinical Immunology 120 (1) : 105-112. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jaci.2007.02.032
dc.identifier.issn00916749
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/26314
dc.description.abstractBackground: Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Objective: We sought to identify and characterize a novel allergen, Blo t 21, from B tropicalis. Methods: Blo t 21 was initially identified from an expressed sequence tag database generated from a B tropicalis cDNA library. Allergenicity of this antigen was examined by means of skin prick testing, ELISA, and IgE immuno-dot blotting. We evaluated whether Blo t 21 and Blo t 5 were cross-reactive by using IgE inhibition ELISAs. Results: Blo t 21, a 129-amino-acid protein sharing 39% identity with Blo t 5, is a product of a single-copy gene. It has an α-helical secondary structure and localizes to midgut and hindgut contents of B tropicalis, as well as fecal particles. Positive responses to Blo t 21 were shown in 93% (40/43) by means of ELISA and 95% (41/43) by means of skin prick testing when assayed in 43 adult patients with ongoing persistent allergic rhinitis. However, sera of 494 consecutive individuals attending outpatient allergy clinics over 11/2 years showed 57.9% (286/494) had positive responses to Blo t 21. Although the majority (>75%) of sensitized individuals were cosensitized to both Blo t 5 and Blo t 21, these 2 allergens had a low-to-moderate degree of cross-reactivity. Conclusion: Blo t 21 is a major allergen in B tropicalis that is not highly cross-reactive to Blo t 5, despite sharing some sequence and structural identity. Clinical implications: Blo t 21, representing a new group of allergens, is an important B tropicalis allergen. © 2007 American Academy of Allergy, Asthma & Immunology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jaci.2007.02.032
dc.sourceScopus
dc.subjectallergen
dc.subjectBlomia tropicalis
dc.subjectcross-reactivity
dc.subjectDust mite
dc.subjectgenomic organization
dc.subjectIgE binding
dc.subjectimmunolocalization
dc.typeArticle
dc.contributor.departmentOTOLARYNGOLOGY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.jaci.2007.02.032
dc.description.sourcetitleJournal of Allergy and Clinical Immunology
dc.description.volume120
dc.description.issue1
dc.description.page105-112
dc.identifier.isiut000248066400015
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