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Title: Identification and characterization of a novel allergen from Blomia tropicalis: Blo t 21
Authors: Gao, Y.F. 
Ong, T.C. 
Tay, S.L.
Yap, K.H.
Chew, F.T. 
Wang, D.Y. 
Keywords: allergen
Blomia tropicalis
Dust mite
genomic organization
IgE binding
Issue Date: 2007
Citation: Gao, Y.F., Ong, T.C., Tay, S.L., Yap, K.H., Chew, F.T., Wang, D.Y. (2007). Identification and characterization of a novel allergen from Blomia tropicalis: Blo t 21. Journal of Allergy and Clinical Immunology 120 (1) : 105-112. ScholarBank@NUS Repository.
Abstract: Background: Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Objective: We sought to identify and characterize a novel allergen, Blo t 21, from B tropicalis. Methods: Blo t 21 was initially identified from an expressed sequence tag database generated from a B tropicalis cDNA library. Allergenicity of this antigen was examined by means of skin prick testing, ELISA, and IgE immuno-dot blotting. We evaluated whether Blo t 21 and Blo t 5 were cross-reactive by using IgE inhibition ELISAs. Results: Blo t 21, a 129-amino-acid protein sharing 39% identity with Blo t 5, is a product of a single-copy gene. It has an α-helical secondary structure and localizes to midgut and hindgut contents of B tropicalis, as well as fecal particles. Positive responses to Blo t 21 were shown in 93% (40/43) by means of ELISA and 95% (41/43) by means of skin prick testing when assayed in 43 adult patients with ongoing persistent allergic rhinitis. However, sera of 494 consecutive individuals attending outpatient allergy clinics over 11/2 years showed 57.9% (286/494) had positive responses to Blo t 21. Although the majority (>75%) of sensitized individuals were cosensitized to both Blo t 5 and Blo t 21, these 2 allergens had a low-to-moderate degree of cross-reactivity. Conclusion: Blo t 21 is a major allergen in B tropicalis that is not highly cross-reactive to Blo t 5, despite sharing some sequence and structural identity. Clinical implications: Blo t 21, representing a new group of allergens, is an important B tropicalis allergen. © 2007 American Academy of Allergy, Asthma & Immunology.
Source Title: Journal of Allergy and Clinical Immunology
ISSN: 00916749
DOI: 10.1016/j.jaci.2007.02.032
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