Please use this identifier to cite or link to this item:
|Title:||Identification and characterization of a novel allergen from Blomia tropicalis: Blo t 21||Authors:||Gao, Y.F.
|Issue Date:||2007||Citation:||Gao, Y.F., Ong, T.C., Tay, S.L., Yap, K.H., Chew, F.T., Wang, D.Y. (2007). Identification and characterization of a novel allergen from Blomia tropicalis: Blo t 21. Journal of Allergy and Clinical Immunology 120 (1) : 105-112. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jaci.2007.02.032||Abstract:||Background: Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Objective: We sought to identify and characterize a novel allergen, Blo t 21, from B tropicalis. Methods: Blo t 21 was initially identified from an expressed sequence tag database generated from a B tropicalis cDNA library. Allergenicity of this antigen was examined by means of skin prick testing, ELISA, and IgE immuno-dot blotting. We evaluated whether Blo t 21 and Blo t 5 were cross-reactive by using IgE inhibition ELISAs. Results: Blo t 21, a 129-amino-acid protein sharing 39% identity with Blo t 5, is a product of a single-copy gene. It has an α-helical secondary structure and localizes to midgut and hindgut contents of B tropicalis, as well as fecal particles. Positive responses to Blo t 21 were shown in 93% (40/43) by means of ELISA and 95% (41/43) by means of skin prick testing when assayed in 43 adult patients with ongoing persistent allergic rhinitis. However, sera of 494 consecutive individuals attending outpatient allergy clinics over 11/2 years showed 57.9% (286/494) had positive responses to Blo t 21. Although the majority (>75%) of sensitized individuals were cosensitized to both Blo t 5 and Blo t 21, these 2 allergens had a low-to-moderate degree of cross-reactivity. Conclusion: Blo t 21 is a major allergen in B tropicalis that is not highly cross-reactive to Blo t 5, despite sharing some sequence and structural identity. Clinical implications: Blo t 21, representing a new group of allergens, is an important B tropicalis allergen. © 2007 American Academy of Allergy, Asthma & Immunology.||Source Title:||Journal of Allergy and Clinical Immunology||URI:||http://scholarbank.nus.edu.sg/handle/10635/26314||ISSN:||00916749||DOI:||10.1016/j.jaci.2007.02.032|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 12, 2021
WEB OF SCIENCETM
checked on Jun 12, 2021
checked on May 29, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.