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|Title:||The search for factors in human feeders that support the derivation and propagation of human embryonic stem cells: preliminary studies using transcriptome profiling by serial analysis of gene expression||Authors:||Kueh, J.
|Issue Date:||2006||Citation:||Kueh, J., Richards, M., Ng, S.-W., Bongso, A., Chan, W.-K. (2006). The search for factors in human feeders that support the derivation and propagation of human embryonic stem cells: preliminary studies using transcriptome profiling by serial analysis of gene expression. Fertility and Sterility 85 (6) : 1843-1846. ScholarBank@NUS Repository.||Abstract:||Serial analysis of gene expression (SAGE) was used to obtain the transcriptome profiles of a supportive human fetal skin feeder (Detroit 551) and a nonsupportive human fetal lung feeder (MRC-5) for human embryonic stem cells. A pairwise comparison of the two SAGE profiles showed that fibroblast growth factor-2 (FGF2), a bone morphogenetic protein 4 pathway inhibitor, Gremlin 1, and several extracellular matrix proteins that could potentially aid human embryonic stem cell attachment and growth were highly expressed in Detroit 551 fibroblasts. © 2006 American Society for Reproductive Medicine.||Source Title:||Fertility and Sterility||URI:||http://scholarbank.nus.edu.sg/handle/10635/26107||ISSN:||00150282|
|Appears in Collections:||Staff Publications|
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