Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jpba.2008.12.036
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dc.titleDetermination of breviflavone A and B in Epimedium herbs with liquid chromatography-tandem mass spectrometry
dc.contributor.authorHong, X.
dc.contributor.authorWang, X.
dc.contributor.authorYong, E.L.
dc.contributor.authorGong, Y.
dc.date.accessioned2011-09-15T08:22:15Z
dc.date.available2011-09-15T08:22:15Z
dc.date.issued2009
dc.identifier.citationHong, X., Wang, X., Yong, E.L., Gong, Y. (2009). Determination of breviflavone A and B in Epimedium herbs with liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 49 (3) : 853-857. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.12.036
dc.identifier.issn07317085
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/26023
dc.description.abstractTwo new types of minor flavonoids, breviflavone A and B, have been recently isolated and identified from Epimedium brevicornu in our previous research. Breviflavone B is a novel flavonoid with potent and specific estrogen receptor (ER) bioactivity. Its positional isomer, breviflavone A, is not ER active. Therefore, it is important to determine the two minor components, breviflavone A and B, in Epimedium herbs. In this report, a robust method for measurement of the two breviflavones in Epimedium ethanolic extracts has been developed by using liquid chromatography tandem mass spectrometry via selected-reaction monitoring (m/z 437 → m/z 367 for breviflavone A and m/z 437 → m/z 351 for breviflavone B) under negative electrospray ionization mode. This method has been successfully used to determine the two breviflavones in ethanolic herbal extracts of five major Epimedium species (E. brevicornu, E. koreanum, E. pubescens, E. sagittatum, and E. wushanese) from various sources. The contents of the two breviflavones range from 0.0181 to 0.1791% for breviflavone A and 0.0026 to 0.0252% for breviflavone B in the dried ethanolic extracts of those Epimedium herbal samples. © 2009 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jpba.2008.12.036
dc.sourceScopus
dc.subjectBreviflavone
dc.subjectEpimedium
dc.subjectLiquid chromatography tandem mass spectrometry
dc.subjectPhytoestrogen
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1016/j.jpba.2008.12.036
dc.description.sourcetitleJournal of Pharmaceutical and Biomedical Analysis
dc.description.volume49
dc.description.issue3
dc.description.page853-857
dc.identifier.isiut000264409800042
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