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Title: WNT Signaling in the early development of Zebrfish Swimbladder and Xenopus Lung
Authors: YIN AO
Keywords: Wnt, swimbladder, zebrafish, IWR-1, wif1, heatshock
Issue Date: 17-Jan-2011
Citation: YIN AO (2011-01-17). WNT Signaling in the early development of Zebrfish Swimbladder and Xenopus Lung. ScholarBank@NUS Repository.
Abstract: Comparative study of lung and swimbladder development is not only an important issue in developmental biology, but also an attractive topic in evolutionary biology. However, although the homology between lung and swimbladder is supported by their common morphological origin and blood supply from the 6th branchial artery, molecular evidence remains largely missing. Preciously, we demonstrated that many genes important for induction of lung bud and early lung development are also expressed in zebrafish swimbladder development. In particular, the hedgehog signaling, essential for lung development, is also required for proper development of all the three tissue layers of swimbladder. Although Wnt signaling pathway has been reported to play a critical role in mammalian lung development, the role of Wnt signaling in zebrafish swimbladder and Xenopus lung development has not been investigated yet. In the current study, we investigated Wnt signaling in the Xenopus and zebrafish models. The expression of sftpc, nkx2.1, wnt7b, wnt5a, wif1 and shh in the early Xenopus lung were demonstrated. In zebrafish, a number of Wnt component genes expressed in the three tissue layers of swimbladder, including wif1, wnt5b, wnt11, axin1, axin2, tcf3, fz2, fz7a, wif1, were also identified. By employing three different approaches to manipulate Wnt signaling, including using the hs:Dkk1-GFP and hs:¿Tcf-GFP transgenic lines which are engineered for heat-shock-inducible Wnt inhibition, the chemical inhibitor of Wnt signaling, IWR-1, and up-regulation of Wnt signaling by knockdown the Wnt protein inhibitor wif1, we demonstrated that Wnt signaling played critical roles in the specification, proliferation, apoptosis inhibition, organization in all the three layers and smooth muscle differentiation in swimbladder. Furthermore, we investigated the roles of Wnt ligands genes wnt1, wnt5b and wnt11 in the early development of zebrafish swimbladder. In addition, the cross-talks between Wnt signaling and Hedgehog signaling as well as tbx2a signaling were investigated. In conclusion, our study demonstrated the roles of Wnt signaling are conserved between the early development of zebrafish swimbladder and mouse lung.
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