Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2010.01.028
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dc.titleDimeric gold nanoparticle assembly for detection and discrimination of single nucleotide mutation in Duchenne muscular dystrophy
dc.contributor.authorQin, W.J.
dc.contributor.authorYung, L.-Y.L.
dc.contributor.authorYim, O.S.
dc.contributor.authorLai, P.S.
dc.date.accessioned2011-08-16T07:53:10Z
dc.date.available2011-08-16T07:53:10Z
dc.date.issued2010
dc.identifier.citationQin, W.J., Yung, L.-Y.L., Yim, O.S., Lai, P.S. (2010). Dimeric gold nanoparticle assembly for detection and discrimination of single nucleotide mutation in Duchenne muscular dystrophy. Biosensors and Bioelectronics 25 (9) : 2021-2025. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2010.01.028
dc.identifier.issn09565663
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25780
dc.description.abstractNanoparticles are increasingly being used for applications in clinical diagnostics due to their unique physical and chemical properties. Gold nanoparticles, in particular, have unique optical properties allowing simplicity of detection methods. In this study, an assay based on dimeric assembly of gold nanoparticles was developed for discriminating single nucleotide mismatches. Only gel electrophoresis is needed for assay readout. No other sophisticated or expensive equipment is required. In addition, no false-positive was observed in the readout. We used this assay for genotyping mutations in the Duchenne muscular dystrophy (DMD) gene, the largest known in the human genome. Our results show that conjugating the gold nanoparticles with short DNA probes of 18 bases and 70 bases complimentary to target sequences allows specific discrimination between wild-type and mutant sequences for c.4150G > T (NM.004006.1) mutation in exon 30 of the DMD gene using a simple colorimetric detection. This method allows identification of both the patients as well as the carriers of the mutation who are at risk of transmitting the disease. © 2010 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bios.2010.01.028
dc.sourceScopus
dc.subjectDNA hybridization
dc.subjectDuchenne muscular dystrophy
dc.subjectGold nanoparticle
dc.subjectSingle nucleotide mutation
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentPAEDIATRICS
dc.description.doi10.1016/j.bios.2010.01.028
dc.description.sourcetitleBiosensors and Bioelectronics
dc.description.volume25
dc.description.issue9
dc.description.page2021-2025
dc.identifier.isiut000277930000004
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