Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.clinbiochem.2007.01.003

Title:	Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection
Authors:	Quek, D.L. Ng, Y.-Y. Tang-Lim, G.-I. Wang, W. Chong, S.S. Tan, A.S.C. Ng, I.S.L. Law, H.-Y.
Keywords:	β-Thalassemia Carrier screening Dissociation curve analysis Mutations SYBR® green
Issue Date:	2007
Citation:	Quek, D.L., Ng, Y.-Y., Tang-Lim, G.-I., Wang, W., Chong, S.S., Tan, A.S.C., Ng, I.S.L., Law, H.-Y. (2007). Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection. Clinical Biochemistry 40 (5-6) : 427-430. ScholarBank@NUS Repository. https://doi.org/10.1016/j.clinbiochem.2007.01.003
Abstract:	Objectives: This study aimed to evaluate a rapid molecular carrier screening strategy for β-thalassemia. Design and methods: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR® Green I fluorescence in a single step. Results: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. Conclusions: Homogeneous allele-specific PCR amplification and detection of multiple β-globin mutations can serve as a rapid and inexpensive carrier screening tool. © 2007 The Canadian Society of Clinical Chemists.
Source Title:	Clinical Biochemistry
URI:	http://scholarbank.nus.edu.sg/handle/10635/25627
ISSN:	00099120
DOI:	10.1016/j.clinbiochem.2007.01.003
Appears in Collections:	Staff Publications

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