Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.clinbiochem.2007.01.003
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dc.titleRapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection
dc.contributor.authorQuek, D.L.
dc.contributor.authorNg, Y.-Y.
dc.contributor.authorTang-Lim, G.-I.
dc.contributor.authorWang, W.
dc.contributor.authorChong, S.S.
dc.contributor.authorTan, A.S.C.
dc.contributor.authorNg, I.S.L.
dc.contributor.authorLaw, H.-Y.
dc.date.accessioned2011-08-16T07:42:07Z
dc.date.available2011-08-16T07:42:07Z
dc.date.issued2007
dc.identifier.citationQuek, D.L., Ng, Y.-Y., Tang-Lim, G.-I., Wang, W., Chong, S.S., Tan, A.S.C., Ng, I.S.L., Law, H.-Y. (2007). Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection. Clinical Biochemistry 40 (5-6) : 427-430. ScholarBank@NUS Repository. https://doi.org/10.1016/j.clinbiochem.2007.01.003
dc.identifier.issn00099120
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25627
dc.description.abstractObjectives: This study aimed to evaluate a rapid molecular carrier screening strategy for β-thalassemia. Design and methods: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR® Green I fluorescence in a single step. Results: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. Conclusions: Homogeneous allele-specific PCR amplification and detection of multiple β-globin mutations can serve as a rapid and inexpensive carrier screening tool. © 2007 The Canadian Society of Clinical Chemists.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.clinbiochem.2007.01.003
dc.sourceScopus
dc.subjectβ-Thalassemia
dc.subjectCarrier screening
dc.subjectDissociation curve analysis
dc.subjectMutations
dc.subjectSYBR® green
dc.typeArticle
dc.contributor.departmentPAEDIATRICS
dc.description.doi10.1016/j.clinbiochem.2007.01.003
dc.description.sourcetitleClinical Biochemistry
dc.description.volume40
dc.description.issue5-6
dc.description.page427-430
dc.identifier.isiut000244997400028
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