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Title: Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection
Authors: Quek, D.L.
Ng, Y.-Y.
Tang-Lim, G.-I.
Wang, W.
Chong, S.S. 
Tan, A.S.C.
Ng, I.S.L.
Law, H.-Y.
Keywords: β-Thalassemia
Carrier screening
Dissociation curve analysis
SYBR® green
Issue Date: 2007
Citation: Quek, D.L., Ng, Y.-Y., Tang-Lim, G.-I., Wang, W., Chong, S.S., Tan, A.S.C., Ng, I.S.L., Law, H.-Y. (2007). Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection. Clinical Biochemistry 40 (5-6) : 427-430. ScholarBank@NUS Repository.
Abstract: Objectives: This study aimed to evaluate a rapid molecular carrier screening strategy for β-thalassemia. Design and methods: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR® Green I fluorescence in a single step. Results: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. Conclusions: Homogeneous allele-specific PCR amplification and detection of multiple β-globin mutations can serve as a rapid and inexpensive carrier screening tool. © 2007 The Canadian Society of Clinical Chemists.
Source Title: Clinical Biochemistry
ISSN: 00099120
DOI: 10.1016/j.clinbiochem.2007.01.003
Appears in Collections:Staff Publications

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