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|Title:||Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection||Authors:||Quek, D.L.
Dissociation curve analysis
|Issue Date:||2007||Citation:||Quek, D.L., Ng, Y.-Y., Tang-Lim, G.-I., Wang, W., Chong, S.S., Tan, A.S.C., Ng, I.S.L., Law, H.-Y. (2007). Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection. Clinical Biochemistry 40 (5-6) : 427-430. ScholarBank@NUS Repository. https://doi.org/10.1016/j.clinbiochem.2007.01.003||Abstract:||Objectives: This study aimed to evaluate a rapid molecular carrier screening strategy for β-thalassemia. Design and methods: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR® Green I fluorescence in a single step. Results: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. Conclusions: Homogeneous allele-specific PCR amplification and detection of multiple β-globin mutations can serve as a rapid and inexpensive carrier screening tool. © 2007 The Canadian Society of Clinical Chemists.||Source Title:||Clinical Biochemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/25627||ISSN:||00099120||DOI:||10.1016/j.clinbiochem.2007.01.003|
|Appears in Collections:||Staff Publications|
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