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Title: Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production
Authors: Tan, L.K. 
Huang, C.-H. 
Liew, L.M.
Chua, K.Y. 
Kuo, I.-C.
Keywords: Allergen vaccination
Dendritic cell
Der p 2
Signal peptide
Issue Date: 2006
Citation: Tan, L.K., Huang, C.-H., Liew, L.M., Chua, K.Y., Kuo, I.-C. (2006). Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production. Vaccine 24 (29-30) : 5762-5771. ScholarBank@NUS Repository.
Abstract: Background: Previous studies demonstrated that allergen gene vaccination induced TH1-skewed responses and inhibited IgE production. This study evaluated and characterized the immune responses induced by three DNA constructs encoding different forms of Der p 2 for safe and efficacious vaccination against mite allergy. Methods: Mice were immunized intramuscularly with DNA constructs encoding a major mite allergen, Der p 2, without a signal peptide (p2), with a signal peptide (p52), and with a signal peptide plus lysosomal-targeting sequence (p52-LA), respectively, followed by TH2-skewed protein challenge. Antibody and T-cell cytokine responses were assessed by ELISA. Primed dendritic cells (DCs) were adoptively transferred to naïve mice and humoral responses were examined after protein challenge. The circulating Der p 2 protein was detected by sandwich ELISA. Results: Mice immunized with p52-LA showed strong and clear-cut TH1-type response, as evident by high IFN-γ production and elevated levels of Der p 2-specific IgG2a production whereas construct p2 induced only moderate levels of TH1 response. In contrast, mice immunized with construct p52 showed a mixed TH1/TH2 phenotype and produced substantial circulating Der p 2 protein. Mice adoptively transferred with DCs primed by p52 construct, but not by the p2 or p52-LA constructs, were sensitized to produce high levels of Der p 2-specific IgE. Conclusions: Immunization with DNA construct encoding a signal peptide could potentially prime TH2-skewed responses and IgE production. The additional inclusion of lysosomal-targeting sequences to such construct could improve the safety and efficacy of DNA vaccination against allergy. © 2006 Elsevier Ltd. All rights reserved.
Source Title: Vaccine
ISSN: 0264410X
DOI: 10.1016/j.vaccine.2006.04.064
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