Please use this identifier to cite or link to this item:
|Title:||Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production||Authors:||Tan, L.K.
Der p 2
|Issue Date:||2006||Citation:||Tan, L.K., Huang, C.-H., Liew, L.M., Chua, K.Y., Kuo, I.-C. (2006). Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production. Vaccine 24 (29-30) : 5762-5771. ScholarBank@NUS Repository. https://doi.org/10.1016/j.vaccine.2006.04.064||Abstract:||Background: Previous studies demonstrated that allergen gene vaccination induced TH1-skewed responses and inhibited IgE production. This study evaluated and characterized the immune responses induced by three DNA constructs encoding different forms of Der p 2 for safe and efficacious vaccination against mite allergy. Methods: Mice were immunized intramuscularly with DNA constructs encoding a major mite allergen, Der p 2, without a signal peptide (p2), with a signal peptide (p52), and with a signal peptide plus lysosomal-targeting sequence (p52-LA), respectively, followed by TH2-skewed protein challenge. Antibody and T-cell cytokine responses were assessed by ELISA. Primed dendritic cells (DCs) were adoptively transferred to naïve mice and humoral responses were examined after protein challenge. The circulating Der p 2 protein was detected by sandwich ELISA. Results: Mice immunized with p52-LA showed strong and clear-cut TH1-type response, as evident by high IFN-γ production and elevated levels of Der p 2-specific IgG2a production whereas construct p2 induced only moderate levels of TH1 response. In contrast, mice immunized with construct p52 showed a mixed TH1/TH2 phenotype and produced substantial circulating Der p 2 protein. Mice adoptively transferred with DCs primed by p52 construct, but not by the p2 or p52-LA constructs, were sensitized to produce high levels of Der p 2-specific IgE. Conclusions: Immunization with DNA construct encoding a signal peptide could potentially prime TH2-skewed responses and IgE production. The additional inclusion of lysosomal-targeting sequences to such construct could improve the safety and efficacy of DNA vaccination against allergy. © 2006 Elsevier Ltd. All rights reserved.||Source Title:||Vaccine||URI:||http://scholarbank.nus.edu.sg/handle/10635/25617||ISSN:||0264410X||DOI:||10.1016/j.vaccine.2006.04.064|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 13, 2019
WEB OF SCIENCETM
checked on Sep 13, 2019
checked on Sep 9, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.