Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.01.081
DC FieldValue
dc.titleBiodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles for stem cell labeling
dc.contributor.authorWang, L.
dc.contributor.authorShuter, B.
dc.contributor.authorNeoh, K.-G.
dc.contributor.authorKang, E.-T.
dc.contributor.authorWang, S.-C.
dc.date.accessioned2011-08-16T05:50:46Z
dc.date.available2011-08-16T05:50:46Z
dc.date.issued2010
dc.identifier.citationWang, L., Shuter, B., Neoh, K.-G., Kang, E.-T., Wang, S.-C. (2010). Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles for stem cell labeling. Biomaterials 31 (13) : 3502-3511. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.01.081
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25575
dc.description.abstractBifunctional superparamagnetic magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles (PLMA-MNPs) detectable by both magnetic resonance imaging (MRI) and fluorescence microscopy were synthesized by coating Fe3O4 nanoparticles with biodegradable poly(dl-lactic acid-co-α,β-malic acid) copolymer (PLMA) with covalently bound fluorescein isothiocyanate (FITC). The FITC modified PLMA-MNPs (FITC-PLMA-MNPs) have a hydrodynamic diameter of 100 nm and an anionic surface. MTT assays of mouse macrophages, 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) incubated with these nanoparticles indicated that these nanoparticles did not possess significant cytotoxicity. Furthermore, the osteogenic and adipogenic differentiations of the hMSCs were not influenced by the labeling process. As a result of the high R2 (164.8 mm-1 s-1) and R2/R1 ratio (32) of the FITC-PLMA-MNPs, the labeled hMSCs can be detected by a clinical 3T MRI scanner at an in vitro detection threshold of about 1200 cells. The green fluorescence associated with the FITC can be readily observed. Such nanoparticles can potentially be used as a T2-weighted contrast agent and fluorescent agent for stem cell labeling. © 2010 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2010.01.081
dc.sourceScopus
dc.subjectBiodegradable
dc.subjectMRI
dc.subjectNanoparticles
dc.subjectPoly(dl-lactic acid-co-α,β-malic acid)
dc.subjectStem cells
dc.typeArticle
dc.contributor.departmentDIAGNOSTIC RADIOLOGY
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biomaterials.2010.01.081
dc.description.sourcetitleBiomaterials
dc.description.volume31
dc.description.issue13
dc.description.page3502-3511
dc.identifier.isiut000276254100009
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