Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2007.11.023
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dc.titleA comparison of rabbit mesenchymal stem cells and anterior cruciate ligament fibroblasts responses on combined silk scaffolds
dc.contributor.authorLiu, H.
dc.contributor.authorFan, H.
dc.contributor.authorGoh, J.C.H.
dc.contributor.authorToh, S.L.
dc.date.accessioned2011-08-03T01:47:19Z
dc.date.available2011-08-03T01:47:19Z
dc.date.issued2008
dc.identifier.citationLiu, H., Fan, H., Goh, J.C.H., Toh, S.L. (2008). A comparison of rabbit mesenchymal stem cells and anterior cruciate ligament fibroblasts responses on combined silk scaffolds. Biomaterials 29 (10) : 1443-1453. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.11.023
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25265
dc.description.abstractThe aim of this study was to compare the cellular responses of bone marrow-derived mesenchymal stem cells (BMSCs) and anterior cruciate ligament fibroblasts (ACLFs) on combined silk scaffolds for ligament tissue engineering application. Rabbit BMSCs and ACLFs were isolated and cultured in vitro for two weeks after seeding on the silk scaffolds. Samples were evaluated and compared for their cellular morphology, proliferation, gene and protein expression of tenascin-C, type I and type III collagen. In addition, the two cell types were transfected with green fluorescent protein (GFP) to trace their fate in the knee joints. Preliminary results comparing cell proliferation indicated that BMSCs proliferated faster than ACLFs. Gene expression of the phenotypic markers measured using real-time reverse transcription polymerase chain reaction (RT-PCR) indicated the transcript levels of BMSCs were significantly increased after two weeks of culture, whereas those of ACLFs had no significant difference. The protein levels and localization were determined by western blotting and immunohistochemical staining, the results showed more production of ligament-related extracellular matrix (ECM) by BMSCs as compared to ACLFs. Moreover, 4 weeks postoperatively, more fluorescent cells were presented in BMSC-loaded constructs than in ACLF-loaded constructs. Therefore, based on the cellular response in vitro and in vivo, BMSCs were found to be a better cell source than ACLFs for the further study of ACL tissue engineering. © 2007 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2007.11.023
dc.sourceScopus
dc.subjectAnterior cruciate ligament fibroblast
dc.subjectCombined silk scaffold
dc.subjectLigament tissue engineering
dc.subjectMesenchymal stem cell
dc.typeArticle
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.description.doi10.1016/j.biomaterials.2007.11.023
dc.description.sourcetitleBiomaterials
dc.description.volume29
dc.description.issue10
dc.description.page1443-1453
dc.identifier.isiut000253406300015
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