Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.06.024
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dc.titleMicrofabricated silicon nitride membranes for hepatocyte sandwich culture
dc.contributor.authorZhang, S.
dc.contributor.authorXia, L.
dc.contributor.authorKang, C.H.
dc.contributor.authorOng, S.M.
dc.contributor.authorToh, Y.C.
dc.contributor.authorLeo, H.L.
dc.contributor.authorvan, Noort D.
dc.contributor.authorKan, S.H.
dc.contributor.authorYu, H.
dc.contributor.authorXiao, G.
dc.contributor.authorTang, H.H.
dc.date.accessioned2011-08-01T03:06:09Z
dc.date.available2011-08-01T03:06:09Z
dc.date.issued2008
dc.identifier.citationZhang, S., Xia, L., Kang, C.H., Ong, S.M., Toh, Y.C., Leo, H.L., van, Noort D., Kan, S.H., Yu, H., Xiao, G., Tang, H.H. (2008). Microfabricated silicon nitride membranes for hepatocyte sandwich culture. Biomaterials 29 (29) : 3993-4002. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.06.024
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25170
dc.description.abstractWe have developed a hepatocyte sandwich culture with improved mass transport properties based on ultra-thin microfabricated porous silicon nitride (Si3N4) membranes. The dimensions and uniformity of the membrane pores can be configurable, which confers more control over the mass transport. Instead of collagen gels used in conventional sandwich culture, we utilized galactose ligands immobilized on the Si3N4 membranes to support hepatocyte attachment and function in the sandwich culture. Diffusion studies using FITC-dextrans confirmed that mass transport of the microfabricated Si3N4 membrane based sandwich was significantly better than conventional collagen gel sandwich and can be configured by varying the porosity of the Si3N4 membrane. Hepatocytes cultured in the microfabricated Si3N4 membrane based sandwich culture exhibited earlier apical repolarization and biliary excretion, improved differentiated functions and enhanced drug sensitivity compared to hepatocytes cultured in a collagen gel sandwich. The Si3N4 membrane based sandwich culture allows for a systematic optimization of the mass transport properties of hepatocyte culture by changing the pore size and inter-pore distance. This will enable more effective drug testing applications where optimal mass transport is required for hepatocyte function maintenance and drug accessibility. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.06.024
dc.sourceScopus
dc.subjectDrug testing
dc.subjectGalactose immobilization
dc.subjectHepatocytes
dc.subjectMass transport
dc.subjectPorous Si3N4membrane
dc.subjectSandwich culture
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/j.biomaterials.2008.06.024
dc.description.sourcetitleBiomaterials
dc.description.volume29
dc.description.issue29
dc.description.page3993-4002
dc.identifier.isiut000259059100010
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