Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2009.07.022
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dc.titleLaminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing
dc.contributor.authorXia, L.
dc.contributor.authorNg, S.
dc.contributor.authorHan, R.
dc.contributor.authorTuo, X.
dc.contributor.authorXiao, G.
dc.contributor.authorLeo, H.L.
dc.contributor.authorCheng, T.
dc.contributor.authorYu, H.
dc.date.accessioned2011-08-01T03:06:04Z
dc.date.available2011-08-01T03:06:04Z
dc.date.issued2009
dc.identifier.citationXia, L., Ng, S., Han, R., Tuo, X., Xiao, G., Leo, H.L., Cheng, T., Yu, H. (2009). Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing. Biomaterials 30 (30) : 5927-5936. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.07.022
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25166
dc.description.abstractDrug hepatotoxicity testing requires in vitro hepatocyte culture to maintain the long-term and stable liver specific functions. We developed a drug testing platform based on laminar-flow immediate-overlay hepatocyte sandwich perfusion culture. The immediate-overlay sandwich (collagen-coated porous polymeric membrane as top overlay) protects the cells and integrity of the top collagen matrix from the impact of flow. A bioreactor was designed that allowed proper control of shear stress and mass transfer. The culture parameters such as the optimal perfusion initiation time and flow rate were systematically and mechanistically determined. The optimized system could re-establish hepatocyte polarity to support biliary excretion and to maintain other liver specific functions, such as the biotransformation enzyme activities, for two weeks that extended the usable in vitro hepatocyte-based drug testing window. When the perfusion cultured hepatocytes from days 7 or 14 were used for drug testing, the APAP-induced hepatotoxicity measurements were more sensitive and consistent over time than the static culture control, enabling further exploitations in large-scale drug testing applications. © 2009 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2009.07.022
dc.sourceScopus
dc.subjectMass transfer
dc.subjectPerfusion bioreactor
dc.subjectPolarity
dc.subjectSandwich culture
dc.subjectShear stress
dc.subjectStable functions
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentDIVISION OF BIOENGINEERING
dc.description.doi10.1016/j.biomaterials.2009.07.022
dc.description.sourcetitleBiomaterials
dc.description.volume30
dc.description.issue30
dc.description.page5927-5936
dc.identifier.isiut000270204500004
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