Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.mrfmmm.2007.06.005
DC Field | Value | |
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dc.title | Inhibition of telomerase activity and human telomerase reverse transcriptase gene expression by histone deacetylase inhibitor in human brain cancer cells | |
dc.contributor.author | Khaw, A.K. | |
dc.contributor.author | Silasudjana, M. | |
dc.contributor.author | Banerjee, B. | |
dc.contributor.author | Hande, M.P. | |
dc.contributor.author | Suzuki, M. | |
dc.contributor.author | Baskar, R. | |
dc.date.accessioned | 2011-08-01T03:04:32Z | |
dc.date.available | 2011-08-01T03:04:32Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Khaw, A.K., Silasudjana, M., Banerjee, B., Hande, M.P., Suzuki, M., Baskar, R. (2007). Inhibition of telomerase activity and human telomerase reverse transcriptase gene expression by histone deacetylase inhibitor in human brain cancer cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 625 (1-2) : 134-144. ScholarBank@NUS Repository. https://doi.org/10.1016/j.mrfmmm.2007.06.005 | |
dc.identifier.issn | 00275107 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/25086 | |
dc.description.abstract | The aim of the present study is to investigate the effect of histone deacetylase inhibitor, trichostatin A (TSA) on the cell growth, apoptosis, genomic DNA damage and the expression of telomerase and associated factors in human normal and brain cancer cells. Here, human normal un-transformed fibroblasts (MRC-5), human normal hTERT-immortalised fibroblasts (hTERT-BJ1) and human brain cancer cell lines (glioblastoma cell line, A-172 and medulloblastoma cell line, ONS-76) were treated with 0.5-3.0 μM TSA for 24 h. Exposure to TSA resulted in apoptosis in a dose-dependent manner in the brain cancer cells. Glioblastoma cell line (A-172) displayed higher sensitivity to TSA-induced cell killing effect and apoptosis than the medulloblastoma cell line (ONS-76). The brain cancer cell lines and hTERT-BJ1 cell line displayed significant inhibition in telomerase activity and hTERT mRNA level after 2 μM TSA treatment. Elevated expressions of p53 and p21 with a decrease in cyclin-D level supported the observation on cell cycle arrest following TSA treatment. Upregulation of Bax and cytochrome c correlated with the apoptotic events in TSA-treated cells. This study suggests that telomerase and hTERT might be the primary targets of TSA which may have the potential to be used as a telomerase inhibitor in cancer therapy. © 2007 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.mrfmmm.2007.06.005 | |
dc.source | Scopus | |
dc.subject | Cancer chemotherapy | |
dc.subject | Genome Instability | |
dc.subject | Glioblastoma and medulloblastoma | |
dc.subject | Histone deacetylase inhibitors | |
dc.subject | Telomeres and telomerase | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | PHARMACOLOGY | |
dc.description.doi | 10.1016/j.mrfmmm.2007.06.005 | |
dc.description.sourcetitle | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis | |
dc.description.volume | 625 | |
dc.description.issue | 1-2 | |
dc.description.page | 134-144 | |
dc.identifier.isiut | 000251467800013 | |
Appears in Collections: | Staff Publications |
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