Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2009.03.052
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dc.titleThe controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system
dc.contributor.authorZhang, C.
dc.contributor.authorOng, S.-M.
dc.contributor.authorZhang, S.
dc.contributor.authorToh, Y.-C.
dc.contributor.authorvan, Noort D.
dc.contributor.authorYu, H.
dc.contributor.authorChia, S.-M.
dc.date.accessioned2011-07-27T06:39:12Z
dc.date.available2011-07-27T06:39:12Z
dc.date.issued2009
dc.identifier.citationZhang, C., Ong, S.-M., Zhang, S., Toh, Y.-C., van, Noort D., Yu, H., Chia, S.-M. (2009). The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system. Biomaterials 30 (23-24) : 3847-3853. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.03.052
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24973
dc.description.abstract3D-microfluidic cell culture systems (3D-μFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100-200 pg/ml TGF-β1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-β1 is achieved by either direct perfusion or in situ controlled release from gelatin microspheres immobilized in the 3D-μFCCS. Primary hepatocytes cultured for 7 days with the in situ controlled released TGF-β1 exhibited up to four-fold higher albumin secretion and two-fold higher phase I/II enzymatic activities, significantly improving the sensitivity of hepatocytes to acetaminophen-mediated hepatotoxicity, compared to hepatocytes cultured with directly perfused TGF-β1 or without TGF-β1. The controlled presentation of TGF-β1 enhanced hepatocyte functions in microfluidic systems without the complications of co-cultures, allowing for simplifications in drug testing and other hepatocyte-based applications. © 2009 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2009.03.052
dc.sourceScopus
dc.subject3D cell constructs
dc.subjectControlled release
dc.subjectHepatocyte co-culture
dc.subjectMicrofluidics
dc.subjectSoluble microenvironment
dc.subjectTransforming growth factor
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.biomaterials.2009.03.052
dc.description.sourcetitleBiomaterials
dc.description.volume30
dc.description.issue23-24
dc.description.page3847-3853
dc.identifier.isiut000267469300006
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