Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.biomaterials.2009.03.052
Title: | The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system | Authors: | Zhang, C. Ong, S.-M. Zhang, S. Toh, Y.-C. van, Noort D. Yu, H. Chia, S.-M. |
Keywords: | 3D cell constructs Controlled release Hepatocyte co-culture Microfluidics Soluble microenvironment Transforming growth factor |
Issue Date: | 2009 | Citation: | Zhang, C., Ong, S.-M., Zhang, S., Toh, Y.-C., van, Noort D., Yu, H., Chia, S.-M. (2009). The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system. Biomaterials 30 (23-24) : 3847-3853. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.03.052 | Abstract: | 3D-microfluidic cell culture systems (3D-μFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100-200 pg/ml TGF-β1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-β1 is achieved by either direct perfusion or in situ controlled release from gelatin microspheres immobilized in the 3D-μFCCS. Primary hepatocytes cultured for 7 days with the in situ controlled released TGF-β1 exhibited up to four-fold higher albumin secretion and two-fold higher phase I/II enzymatic activities, significantly improving the sensitivity of hepatocytes to acetaminophen-mediated hepatotoxicity, compared to hepatocytes cultured with directly perfused TGF-β1 or without TGF-β1. The controlled presentation of TGF-β1 enhanced hepatocyte functions in microfluidic systems without the complications of co-cultures, allowing for simplifications in drug testing and other hepatocyte-based applications. © 2009 Elsevier Ltd. All rights reserved. | Source Title: | Biomaterials | URI: | http://scholarbank.nus.edu.sg/handle/10635/24973 | ISSN: | 01429612 | DOI: | 10.1016/j.biomaterials.2009.03.052 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.