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Title: The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system
Authors: Zhang, C. 
Ong, S.-M.
Zhang, S. 
Toh, Y.-C.
van, Noort D.
Yu, H. 
Chia, S.-M. 
Keywords: 3D cell constructs
Controlled release
Hepatocyte co-culture
Soluble microenvironment
Transforming growth factor
Issue Date: 2009
Citation: Zhang, C., Ong, S.-M., Zhang, S., Toh, Y.-C., van, Noort D., Yu, H., Chia, S.-M. (2009). The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system. Biomaterials 30 (23-24) : 3847-3853. ScholarBank@NUS Repository.
Abstract: 3D-microfluidic cell culture systems (3D-μFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100-200 pg/ml TGF-β1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-β1 is achieved by either direct perfusion or in situ controlled release from gelatin microspheres immobilized in the 3D-μFCCS. Primary hepatocytes cultured for 7 days with the in situ controlled released TGF-β1 exhibited up to four-fold higher albumin secretion and two-fold higher phase I/II enzymatic activities, significantly improving the sensitivity of hepatocytes to acetaminophen-mediated hepatotoxicity, compared to hepatocytes cultured with directly perfused TGF-β1 or without TGF-β1. The controlled presentation of TGF-β1 enhanced hepatocyte functions in microfluidic systems without the complications of co-cultures, allowing for simplifications in drug testing and other hepatocyte-based applications. © 2009 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2009.03.052
Appears in Collections:Staff Publications

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