Please use this identifier to cite or link to this item:
Title: Detection of telomerase activity in gastric lavage fluid: A novel method to detect gastric cancer
Authors: Ching-Ho, Wong S.
Yu, H. 
So, J.B.Y.
Keywords: Gastric cancer
Gastric lavage fluid
Helicobacter pylori
Telomerase activity
Issue Date: 2006
Citation: Ching-Ho, Wong S., Yu, H., So, J.B.Y. (2006). Detection of telomerase activity in gastric lavage fluid: A novel method to detect gastric cancer. Journal of Surgical Research 131 (2) : 252-255. ScholarBank@NUS Repository.
Abstract: Background. Telomerase is a ribonucleoprotein polymerase that is essential for cell immortality. Recent studies have demonstrated that a high percentage of gastric cancer tissue expressed telomerase. This study describes the presence of telomerase activity in gastric lavage fluid in patients with gastric cancer. Methods. Gastric lavage fluid was collected during esophageogastroduodenoscopy in 70 patients: 25 with gastric cancer, 25 with peptic ulcer disease, and 20 with normal stomach. The fluid and biopsy samples were analyzed for telomerase activity by a polymerase chain reaction-based telomerase repeat amplification protocol. The findings were related to the histological results. Results. Telomerase activity was present in 24 of the 25 (96%) gastric cancer tissue and in 7 of the 25 tissue specimens from peptic ulcer or gastritis. In the gastric lavage fluid, telomerase was detected in 20 patients (80%) with gastric cancer, 7 patients (28%) with peptic ulcer, and none in normal subjects (P < 0.001). The sensitivity, specificity, positive predictive value, and negative predictive value of gastric fluid telomerase expression in gastric cancer patients was 80%, 84%, 74%, and 88%, respectively. Conclusions. The presence of telomerase activity is present in gastric lavage fluid of patients with gastric cancer as compared to those without, may represent a novel method for diagnosis of gastric cancer. © 2006 Elsevier Inc. All rights reserved.
Source Title: Journal of Surgical Research
ISSN: 00224804
DOI: 10.1016/j.jss.2005.11.567
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Aug 12, 2022


checked on Aug 12, 2022

Page view(s)

checked on Aug 18, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.