Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.10.062
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dc.titleThe co-delivery of paclitaxel and Herceptin using cationic micellar nanoparticles
dc.contributor.authorLee, A.L.Z.
dc.contributor.authorWang, Y.
dc.contributor.authorCheng, H.Y.
dc.contributor.authorYang, Y.Y.
dc.contributor.authorPervaiz, S.
dc.date.accessioned2011-07-27T06:38:13Z
dc.date.available2011-07-27T06:38:13Z
dc.date.issued2009
dc.identifier.citationLee, A.L.Z., Wang, Y., Cheng, H.Y., Yang, Y.Y., Pervaiz, S. (2009). The co-delivery of paclitaxel and Herceptin using cationic micellar nanoparticles. Biomaterials 30 (5) : 919-927. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.10.062
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24911
dc.description.abstractWe have recently reported micellar nanoparticles self-assembled from a biodegradable and amphiphilic copolymer poly{(N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate}, P(MDS-co-CES), which were able to deliver small molecular drugs and biomacromolecules such as genes and functional proteins individually or simultaneously into various types of cells. In this study, these cationic micellar nanoparticles were employed as carriers to co-deliver paclitaxel and Herceptin for achieving targeted delivery of paclitaxel to human epidermal growth factor receptor-2 (HER2/neu)-overexpressing human breast cancer cells, and enhanced cytotoxicity through synergistic activities. Paclitaxel-loaded nanoparticles have an average size less than 120 nm and a zeta potential of about 60 mV. Herceptin was complexed onto the surface of the nanoparticles. The drug-loaded nanoparticle/Herceptin complexes remained stable under physiologically-simulating conditions with sizes at around 200 nm. The nanoparticles delivered Herceptin much more efficiently than BioPorter, a commercially available lipid-based protein carrier, and displayed a much higher anti-cancer effectiveness. Twice-repeated daily treatment with Herceptin showed significantly higher cytotoxicity especially in HER2-overexpressing breast cancer cells when compared to single treatment. Anti-cancer effects of this co-delivery system was investigated in human breast cancer cell lines with varying degrees of HER2 expression level, namely, MCF7, T47D and BT474. The co-delivery of Herceptin increased the cytotoxicity of paclitaxel and this enhancement showed a dependency on their HER2 expression levels. Targeting ability of this co-delivery system was demonstrated through confocal images, which showed significantly higher cellular uptake in HER2-overexpressing BT474 cells as compared to HER2-negative HEK293 cells. This co-delivery system may have important clinical implications against HER2-overexpressing breast cancers. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.10.062
dc.sourceScopus
dc.subjectAmphiphilic copolymer
dc.subjectCationic micellar nanoparticles
dc.subjectCo-delivery
dc.subjectHerceptin
dc.subjectPaclitaxel
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.biomaterials.2008.10.062
dc.description.sourcetitleBiomaterials
dc.description.volume30
dc.description.issue5
dc.description.page919-927
dc.identifier.isiut000262552600027
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