Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.biocel.2008.05.009
DC Field | Value | |
---|---|---|
dc.title | Oxidative damage induced genotoxic effects in human fibroblasts from Xeroderma Pigmentosum group A patients | |
dc.contributor.author | Low, G.K.M. | |
dc.contributor.author | Fok, E.D.Z. | |
dc.contributor.author | Ting, A.P.L. | |
dc.contributor.author | Hande, M.P. | |
dc.date.accessioned | 2011-07-27T06:38:12Z | |
dc.date.available | 2011-07-27T06:38:12Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Low, G.K.M., Fok, E.D.Z., Ting, A.P.L., Hande, M.P. (2008). Oxidative damage induced genotoxic effects in human fibroblasts from Xeroderma Pigmentosum group A patients. International Journal of Biochemistry and Cell Biology 40 (11) : 2583-2595. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biocel.2008.05.009 | |
dc.identifier.issn | 13572725 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/24910 | |
dc.description.abstract | Xeroderma Pigmentosum A protein plays a pivotal role in the nucleotide excision repair pathway. Through site-directed binding of rigidly kinked double-stranded DNA, it verifies damaged DNA for subsequent excision and incision. Although Xeroderma Pigmentosum A-deficient cells have shown to be defective in oxidative base-lesion repair, the effects of oxidative assault on such cells have not been fully explored. Therefore, we sought to determine the involvement of Xeroderma Pigmentosum A in oxidative DNA damage-repair by treating primary fibroblasts from a patient suffering from Xeroderma Pigmentosum A with sodium arsenite and hydrogen peroxide. Our results show dose-dependent increase in genotoxicity with little change in cytotoxicity with both arsenite and H2O2 in Xeroderma Pigmentosum A-deficient cells compared to control cells. Xeroderma Pigmentosum A-deficient cells displayed increased susceptibility and reduced repair capacity when subjected to DNA damage induced by oxidative stress. Superarray results of apoptotic genes revealed differential expression of ∼10 genes between Xeroderma Pigmentosum A-deficient and normal cells following arsenite treatment. Interestingly, we noted that arsenite did not inflict as much damage in the cells compared to H2O2. Lack of functional Xeroderma Pigmentosum A seems to increase the susceptibility of oxidative stress-induced genotoxicity while retaining cell viability posing as a potential cancer risk factor of Xeroderma Pigmentosum A patients. © 2008 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biocel.2008.05.009 | |
dc.source | Scopus | |
dc.subject | Genome instability | |
dc.subject | Nucleotide excision repair | |
dc.subject | Oxidative DNA damage | |
dc.subject | Telomeres | |
dc.subject | Xeroderma Pigmentosum A | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.1016/j.biocel.2008.05.009 | |
dc.description.sourcetitle | International Journal of Biochemistry and Cell Biology | |
dc.description.volume | 40 | |
dc.description.issue | 11 | |
dc.description.page | 2583-2595 | |
dc.identifier.isiut | 000259134100028 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
SCOPUSTM
Citations
20
checked on Jan 21, 2021
WEB OF SCIENCETM
Citations
18
checked on Jan 13, 2021
Page view(s)
186
checked on Jan 20, 2021
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.