Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.virol.2008.08.002
DC FieldValue
dc.titleProtective immunity against influenza H5N1 virus challenge in mice by intranasal co-administration of baculovirus surface-displayed HA and recombinant CTB as an adjuvant
dc.contributor.authorPrabakaran, M.
dc.contributor.authorVelumani, S.
dc.contributor.authorHe, F.
dc.contributor.authorKaruppannan, A.K.
dc.contributor.authorKwang, J.
dc.contributor.authorGeng, G.Y.
dc.contributor.authorYin, L.K.
dc.date.accessioned2011-07-26T07:05:48Z
dc.date.available2011-07-26T07:05:48Z
dc.date.issued2008
dc.identifier.citationPrabakaran, M., Velumani, S., He, F., Karuppannan, A.K., Kwang, J., Geng, G.Y., Yin, L.K. (2008). Protective immunity against influenza H5N1 virus challenge in mice by intranasal co-administration of baculovirus surface-displayed HA and recombinant CTB as an adjuvant. Virology 380 (2) : 412-420. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2008.08.002
dc.identifier.issn00426822
dc.identifier.issn10960341
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24876
dc.description.abstractThe increasing number of recent outbreaks of HPAI H5N1 in birds and humans brings out an urgent need to develop potent H5N1 vaccine regimens. Here we present a study on the intranasal vaccination of recombinant baculovirus surface-displayed hemagglutinin (BacHA) or inactivated whole H5N1 viral (IWV) vaccine with a recombinant cholera toxin B subunit (rCTB) as a mucosal adjuvant in a BALB/c mouse model. Two groups of mice were vaccinated with different doses (HA titer of log 24 or log 28) of either HA surface-displayed baculovirus or inactivated whole viral vaccine virus adjuvanted with different doses (2 μg or 10 μg) of rCTB. The vaccinations were repeated after 28 days. HA specific serum IgG and mucosal IgA antibodies were quantified by indirect ELISA, and serum neutralizing antibody titer were estimated by hemagglutination inhibition (HI) assay and virus neutralization titer assay. Functional protective efficacy of the vaccine was assessed by host challenge against HPAI H5N1 strains. The results revealed that mice co-administered with log 28 HA titer of BacHA vaccine and adjuvanted with 10 μg of rCTB had a significantly enhanced serum IgG and mucosal IgA immune response and serum microneutralization titer compared with mice administered with unadjuvanted log 24 or log 28 HA titer of BacHA alone. Also vaccination with 10 μg of rCTB and log 28 HA titer of BacHA elicited higher HA specific serum and mucosal antibody levels and serum HI titer than vaccination with log 28 HA titer of inactivated H5N1 virus adjuvanted with the same dose of rCTB. The host challenge study also showed that 10 μg rCTB combined with log 28 HA titer of BacHA provided 100% protection against 10MLD50 of homologous and heterologous H5N1 strains. The study shows that the combination of rH5 HA expressed on baculovirus surface and rCTB mucosal adjuvant form an effective mucosal vaccine against H5N1 infection. This baculovirus surface-displayed vaccine is more efficacious than inactivated H5N1 influenza vaccine when administered by intranasal route and has no biosafety concerns associated with isolation, purification and production of the latter vaccine. © 2008 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.virol.2008.08.002
dc.sourceScopus
dc.subjectH5N1 influenza
dc.subjectHemagglutinin
dc.subjectMucosal vaccine
dc.subjectrCTB
dc.subjectRecombinant baculovirus
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/j.virol.2008.08.002
dc.description.sourcetitleVirology
dc.description.volume380
dc.description.issue2
dc.description.page412-420
dc.identifier.isiut000260148400029
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

58
checked on Sep 29, 2022

WEB OF SCIENCETM
Citations

56
checked on Sep 29, 2022

Page view(s)

380
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.