Please use this identifier to cite or link to this item:
|Title:||An evolutionarily conserved 16-kDa thioredoxin-related protein is an antioxidant which regulates the NF-κB signaling pathway||Authors:||Wang, X.W.
Horseshoe crab thioredoxin-related protein
|Issue Date:||2007||Citation:||Wang, X.W., Liou, Y.-C., Ding, J.L., Ho, B. (2007). An evolutionarily conserved 16-kDa thioredoxin-related protein is an antioxidant which regulates the NF-κB signaling pathway. Free Radical Biology and Medicine 42 (2) : 247-259. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2006.10.040||Abstract:||Thioredoxin (TRX) is generally a 12-kDa protein-disulfide reductase. Here, we report the discovery of a 16-kDa thioredoxin-related protein designated Cr-TRP16, from a "living fossil," the horseshoe crab (Carcinoscorpius rotundicauda). Cr-TRP16 contains an atypical WCPPC catalytic motif and possesses the classical thiodisulfide reductase activity, as indicated by the insulin reduction assay. Furthermore, Cr-TRP16 can function as an antioxidant and protect against DNA nicking by reactive oxygen species. Overexpression of Cr-TRP16 regulated the transcription of NF-κB-dependent genes probably by enhancing NF-κB DNA-binding activity, suggesting possible roles for Cr-TRP16 in modulating the NF-κB signaling pathway. In vivo, the antioxidant pyrrolidine dithiocarbamate suppressed the expression of NF-κB-regulated genes such as IκB and inducible nitric oxide synthase. This further supports the notion that oxidative stress is also a regulatory factor of the NF-κB signaling pathway, a phenomenon which has been entrenched for several hundred million years. Furthermore, we demonstrated that the 16-kDa thioredoxins are evolutionarily conserved from Caenorhabditis elegans to human. Interestingly, thioredoxin-like 6, a human homologue of Cr-TRP16, could also enhance NF-κB DNA-binding activity, suggesting that the regulatory role of the 16-kDa thioredoxins on NF-κB is well conserved through evolution. © 2006 Elsevier Inc. All rights reserved.||Source Title:||Free Radical Biology and Medicine||URI:||http://scholarbank.nus.edu.sg/handle/10635/24872||ISSN:||08915849||DOI:||10.1016/j.freeradbiomed.2006.10.040|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Apr 23, 2019
WEB OF SCIENCETM
checked on Apr 23, 2019
checked on Apr 21, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.