Please use this identifier to cite or link to this item:
|Title:||RNA interference against Enterovirus 71 infection||Authors:||Sim, A.C.N.
|Issue Date:||2005||Citation:||Sim, A.C.N., Luhur, A., Chow, V.T.K., Poh, C.L., Tan, T.M.C. (2005). RNA interference against Enterovirus 71 infection. Virology 341 (1) : 72-79. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2005.06.047||Abstract:||Enterovirus 71 (EV71) is a highly infectious major causative agent of hand, foot, and mouth disease (HFMD) which could lead to severe neurological complications. There is currently no effective therapy against EV71. In this study, RNA interference (RNAi) is employed as a therapeutic approach for specific viral inhibition. Various regions of the EV71 genome were targeted for inhibition by chemically synthesized siRNAs. Transfection of rhabdomyosarcoma (RD) cells with siRNA targeting the 3′UTR, 2C, 3C, or 3D region significantly alleviated cytopathic effects of EV71. The inhibitory effect was dosage-dependent with a corresponding decrease in viral RNA, viral proteins, and plaque formations by EV71. Viral inhibition of siRNA transfected RD cells was still evident after 48 h. In addition, no significant adverse off-target silencing effects were observed. These results demonstrated the potential and feasibility for the use of siRNA as an antiviral therapy for EV71 infections. © 2005 Elsevier Inc. All rights reserved.||Source Title:||Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/24828||ISSN:||00426822||DOI:||10.1016/j.virol.2005.06.047|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 26, 2020
WEB OF SCIENCETM
checked on Oct 19, 2020
checked on Oct 25, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.