Please use this identifier to cite or link to this item:
https://doi.org/10.1074/jbc.M110.123729
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dc.title | The E3 Ubiquitin Ligase IDOL Induces the Degradation of the Low Density Lipoprotein Receptor Family Members VLDLR and ApoER2 | |
dc.contributor.author | Hong, Cynthia | |
dc.contributor.author | Duit, Sarah | |
dc.contributor.author | Jalonen, Pilvi | |
dc.contributor.author | Out, Ruud | |
dc.contributor.author | Scheer, Lilith | |
dc.contributor.author | Sorrentino, Vincenzo | |
dc.contributor.author | Boyadjian, Rima | |
dc.contributor.author | Rodenburg, Kees W | |
dc.contributor.author | Foley, Edan | |
dc.contributor.author | Korhonen, Laura | |
dc.contributor.author | Lindholm, Dan | |
dc.contributor.author | Nimpf, Johannes | |
dc.contributor.author | van Berkel, Theo JC | |
dc.contributor.author | Tontonoz, Peter | |
dc.contributor.author | Zelcer, Noam | |
dc.date.accessioned | 2024-04-11T09:37:24Z | |
dc.date.available | 2024-04-11T09:37:24Z | |
dc.date.issued | 2010-06-25 | |
dc.identifier.citation | Hong, Cynthia, Duit, Sarah, Jalonen, Pilvi, Out, Ruud, Scheer, Lilith, Sorrentino, Vincenzo, Boyadjian, Rima, Rodenburg, Kees W, Foley, Edan, Korhonen, Laura, Lindholm, Dan, Nimpf, Johannes, van Berkel, Theo JC, Tontonoz, Peter, Zelcer, Noam (2010-06-25). The E3 Ubiquitin Ligase IDOL Induces the Degradation of the Low Density Lipoprotein Receptor Family Members VLDLR and ApoER2. JOURNAL OF BIOLOGICAL CHEMISTRY 285 (26) : 19720-19726. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M110.123729 | |
dc.identifier.issn | 0021-9258 | |
dc.identifier.issn | 1083-351X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/247848 | |
dc.description.abstract | We have previously identified the E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor (LDLR) (Idol) as a post-translational modulator of LDLR levels. Idol is a direct target for regulation by liver X receptors (LXRs), and its expression is responsive to cellular sterol status independent of the sterol-response element-binding proteins. Here we demonstrate that Idol also targets two closely related LDLR family members, VLDLR and ApoE receptor 2 (ApoER2), proteins implicated in both neuronal development and lipid metabolism. Idol triggers ubiquitination of the VLDLR and ApoER2 on their cytoplasmic tails, leading to their degradation. We further show that the level of endogenous VLDLR is sensitive to cellular sterol content, Idol expression, and activation of the LXR pathway. Pharmacological activation of the LXR pathway in mice leads to increased Idol expression and to decreased Vldlr levels in vivo. Finally, we establish an unexpected functional link between LXR and Reelin signaling. We demonstrate that LXR activation results indecreased Reelin binding to VLDLR and reduced Dab1 phosphorylation. The identification of VLDLR and ApoER2 as Idol targets suggests potential roles for this LXR-inducible E3 ligase in the central nervous system in addition to lipid metabolism. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. | |
dc.language.iso | en | |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | LIVER-X-RECEPTORS | |
dc.subject | LEUCINE ZIPPER PROTEIN | |
dc.subject | LDL RECEPTOR | |
dc.subject | BRAIN | |
dc.subject | HYPERCHOLESTEROLEMIA | |
dc.subject | REELIN | |
dc.subject | PCSK9 | |
dc.subject | MICE | |
dc.subject | GENE | |
dc.subject | MUTATIONS | |
dc.type | Article | |
dc.date.updated | 2024-04-08T10:56:01Z | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1074/jbc.M110.123729 | |
dc.description.sourcetitle | JOURNAL OF BIOLOGICAL CHEMISTRY | |
dc.description.volume | 285 | |
dc.description.issue | 26 | |
dc.description.page | 19720-19726 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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The E3 ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2.pdf | 971.45 kB | Adobe PDF | OPEN | Published | View/Download |
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