Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.micinf.2010.04.005
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dc.titleWest Nile virus capsid protein interaction with importin and HDM2 protein is regulated by protein kinase C-mediated phosphorylation
dc.contributor.authorBhuvanakantham, R.
dc.contributor.authorCheong, Y.K.
dc.contributor.authorNg, M.-L.
dc.date.accessioned2011-07-26T06:54:21Z
dc.date.available2011-07-26T06:54:21Z
dc.date.issued2010
dc.identifier.citationBhuvanakantham, R., Cheong, Y.K., Ng, M.-L. (2010). West Nile virus capsid protein interaction with importin and HDM2 protein is regulated by protein kinase C-mediated phosphorylation. Microbes and Infection 12 (8-9) : 615-625. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micinf.2010.04.005
dc.identifier.issn12864579
dc.identifier.issn1769714X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24767
dc.description.abstractWest Nile virus (WNV) capsid (C) protein was shown to enter the nucleus via importin-mediated pathway and induce apoptosis although the precise regulatory mechanisms for such events have remained elusive. In this study, it was shown that WNV C protein was phosphorylated by protein kinase C (PKC). PKC-mediated phosphorylation influenced nuclear trafficking of C protein by modulating the efficiency of C protein-importin-α binding. Combination of bio-informatics, site-directed mutagenesis, co-immunoprecipitation, immuno-fluorescence and mammalian two-hybrid analyses showed that phosphorylation at amino acid residues residing near (Ser83) or within (Ser99 and Thr100) the bipartite nuclear localization motif of WNV C protein was essential for efficient interaction between C protein and importin-α. In addition, phosphorylation of WNV C protein by PKC was shown to enhance its binding to HDM2 and could subsequently induce p53-dependent apoptosis. Collectively, this study highlighted that phosphorylation is an important post-translational modification required to execute the functions of C protein. © 2010 Elsevier Masson SAS. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.micinf.2010.04.005
dc.sourceScopus
dc.subjectApoptosis
dc.subjectCapsid
dc.subjectHDM2
dc.subjectImportin
dc.subjectPhosphorylation
dc.subjectPKC
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.micinf.2010.04.005
dc.description.sourcetitleMicrobes and Infection
dc.description.volume12
dc.description.issue8-9
dc.description.page615-625
dc.identifier.isiut000280780100004
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