Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.micinf.2006.09.009
DC FieldValue
dc.titleThe envelope glycoprotein domain III of dengue virus serotypes 1 and 2 inhibit virus entry
dc.contributor.authorChin, J.F.L.
dc.contributor.authorChu, J.J.H.
dc.contributor.authorNg, M.L.
dc.date.accessioned2011-07-26T06:54:18Z
dc.date.available2011-07-26T06:54:18Z
dc.date.issued2007
dc.identifier.citationChin, J.F.L., Chu, J.J.H., Ng, M.L. (2007). The envelope glycoprotein domain III of dengue virus serotypes 1 and 2 inhibit virus entry. Microbes and Infection 9 (1) : 1-6. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micinf.2006.09.009
dc.identifier.issn12864579
dc.identifier.issn1769714X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24764
dc.description.abstractDengue virus (DV) is a flavivirus and its urban transmission is maintained largely by its mosquito vectors and vertebrate host, often human. In this study, investigation was carried out on the involvement of domain III of the envelope (E) glycosylated protein of dengue virus serotypes 1 and 2 (DV-1 and DV-2 DIII) in binding to host cell surfaces, thus mediating virus entry. Domain III protein of flavivirus can also serve as an attractive target in inhibiting virus entry. The respective DV DIII proteins were expressed as soluble recombinant fusion proteins before purification through enzymatic cleavage and affinity purification. The purified recombinant DV-1 and DV-2 DIII proteins both demonstrated the ability to inhibit the entry of DV-1 and DV-2 into HepG2 cells and C6/36 mosquito cells. As such, the DV DIII protein is indeed important for the interaction with cellular receptors in both human and mosquito cells. In addition, this protein induced antibodies that completely neutralized homologous dengue serotypes although not with the same efficiency among the heterologous serotypes. This observation may be of importance when formulating a generic vaccine that is effective against all dengue virus serotypes. © 2006 Elsevier Masson SAS. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.micinf.2006.09.009
dc.sourceScopus
dc.subjectFlavivirus
dc.subjectNeutralizing antibodies
dc.subjectVaccine
dc.subjectVirus entry
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.micinf.2006.09.009
dc.description.sourcetitleMicrobes and Infection
dc.description.volume9
dc.description.issue1
dc.description.page1-6
dc.identifier.isiut000244586400001
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