Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2005.12.133
DC FieldValue
dc.titleSuppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein
dc.contributor.authorLu, L.
dc.contributor.authorHo, Y.
dc.contributor.authorKwang, J.
dc.date.accessioned2011-07-26T06:54:06Z
dc.date.available2011-07-26T06:54:06Z
dc.date.issued2006
dc.identifier.citationLu, L., Ho, Y., Kwang, J. (2006). Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein. Biochemical and Biophysical Research Communications 340 (4) : 1178-1183. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2005.12.133
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24751
dc.description.abstractThe ability to synthesize shRNAs from DNA templates driven by RNA polymerase III promoters has made it possible to apply virus-derived vectors as delivery vehicles for double-strand RNA-mediated interference. Baculovirus emerges as a promising vector for in vivo gene therapy most recently. To investigate its potential as a delivery vector for anti-virus shRNA targeting arterivirus porcine reproductive and respiratory syndrome virus (PRRSV), we constructed recombinant baculovirus vectors bearing a shRNA-synthesizing cassette driven by U6 promoter with enhanced transduction efficiency by displaying vesicular stomatitis virus glycoprotein on viral envelope. Transduction of Marc145 cells with a recombinant baculovirus delivering egfp gene-specific shRNA dramatically suppressed the expression of EGFP in this cell line; and transduction of Marc145 cells with a baculovirus delivering shRNA specific for the C-terminal nucleoprotein coding region of PRRSV genome resulted in inhibition of viral replication. Our data highlight the recombinant baculovirus as an alternative vehicle for anti-virus shRNA delivery. © 2005 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2005.12.133
dc.sourceScopus
dc.subjectBaculovirus
dc.subjectMarc145
dc.subjectPRRSV
dc.subjectRNAi
dc.subjectSF9
dc.subjectshRNA
dc.subjectVSV G
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.bbrc.2005.12.133
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume340
dc.description.issue4
dc.description.page1178-1183
dc.identifier.isiut000235041500023
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