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Title: Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein
Authors: Lu, L.
Ho, Y.
Kwang, J. 
Keywords: Baculovirus
Issue Date: 2006
Citation: Lu, L., Ho, Y., Kwang, J. (2006). Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein. Biochemical and Biophysical Research Communications 340 (4) : 1178-1183. ScholarBank@NUS Repository.
Abstract: The ability to synthesize shRNAs from DNA templates driven by RNA polymerase III promoters has made it possible to apply virus-derived vectors as delivery vehicles for double-strand RNA-mediated interference. Baculovirus emerges as a promising vector for in vivo gene therapy most recently. To investigate its potential as a delivery vector for anti-virus shRNA targeting arterivirus porcine reproductive and respiratory syndrome virus (PRRSV), we constructed recombinant baculovirus vectors bearing a shRNA-synthesizing cassette driven by U6 promoter with enhanced transduction efficiency by displaying vesicular stomatitis virus glycoprotein on viral envelope. Transduction of Marc145 cells with a recombinant baculovirus delivering egfp gene-specific shRNA dramatically suppressed the expression of EGFP in this cell line; and transduction of Marc145 cells with a baculovirus delivering shRNA specific for the C-terminal nucleoprotein coding region of PRRSV genome resulted in inhibition of viral replication. Our data highlight the recombinant baculovirus as an alternative vehicle for anti-virus shRNA delivery. © 2005 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2005.12.133
Appears in Collections:Staff Publications

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