Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.virol.2008.10.024
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dc.titleAttenuation of porcine circovirus 2 in SPF piglets by abrogation of ORF3 function
dc.contributor.authorKaruppannan, A.K.
dc.contributor.authorZhu, Y.
dc.contributor.authorSelvaraj, M.
dc.contributor.authorLau, J.
dc.contributor.authorJia, Q.
dc.contributor.authorKwang, J.
dc.contributor.authorJong, M.H.
dc.contributor.authorLee, S.-H.
dc.date.accessioned2011-07-26T06:54:04Z
dc.date.available2011-07-26T06:54:04Z
dc.date.issued2009
dc.identifier.citationKaruppannan, A.K., Zhu, Y., Selvaraj, M., Lau, J., Jia, Q., Kwang, J., Jong, M.H., Lee, S.-H. (2009). Attenuation of porcine circovirus 2 in SPF piglets by abrogation of ORF3 function. Virology 383 (2) : 338-347. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2008.10.024
dc.identifier.issn00426822
dc.identifier.issn10960341
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24748
dc.description.abstractPorcine circovirus 2 (PCV2), open reading frame 3 (ORF3) codes a 105 amino acid protein that causes apoptosis of PCV2 infected cells. In infected cells, the ORF3 causes the accumulation of p53 by interacting with pPirh2 and possibly by disrupting the association of p53 and pPirh2 (J.Virol.81(2007)9560). Mutant PCV2 lacking the expression of ORF3 are infectious and replicate in cells in vitro, but do not cause apoptosis of the infected cells. The ORF3 of PCV2 has been shown to be involved in pathogenesis of the virus in mice model (J. Virol. 80(2006)5065). Here we report the experimental inoculation of ORF3 deficient PCV2 in its natural host, the piglets. The pathogenicity of the ORF3 deficient virus is attenuated in the piglets. The mutant virus did not cause any observable disease or perturbation of the lymphocyte count in the inoculated piglets and elicited an efficient immune response. When compared with the wildtype virus infection, the mutant virus infection was characterized by mild viremia and absence of pathological lesions. The findings highlight the role of ORF3 in the pathogenesis of PCV2 infection in its host. © 2008 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.virol.2008.10.024
dc.sourceScopus
dc.subjectImmune response
dc.subjectORF3 mutant
dc.subjectPathogenesis
dc.subjectPCV2
dc.subjectSPF piglets
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.virol.2008.10.024
dc.description.sourcetitleVirology
dc.description.volume383
dc.description.issue2
dc.description.page338-347
dc.identifier.isiut000262605700019
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