Please use this identifier to cite or link to this item:
Title: Pfnek3: An atypical activator of a MAP kinase in Plasmodium falciparum
Authors: Lye, Y.M.
Chan, M. 
Sim, T.-S. 
Keywords: Erythrocytic-stage
Protein kinase
Issue Date: 2006
Citation: Lye, Y.M., Chan, M., Sim, T.-S. (2006). Pfnek3: An atypical activator of a MAP kinase in Plasmodium falciparum. FEBS Letters 580 (26) : 6083-6092. ScholarBank@NUS Repository.
Abstract: The canonical mitogen-activated protein kinase (MAPK) signal cascade was previously suggested to be atypical in the malaria parasite. This raises queries on the existence of alternative mediators of plasmodial MAPK pathways. This study describes, Pfnek3, a malarial protein kinase belonging to the NIMA (Never in Mitosis, Aspergillus) family. Endogenous Pfnek3 is expressed during late asexual to gametocyte stages and lacks some classical protein kinase sequence motifs. Moreover, Pfnek3 is phylogenetically distant from mammalian NIMA-kinases. Recombinant Pfnek3 was able to phosphorylate and stimulate a malarial MAPK (Pfmap2). Contrastingly, this was not observed with two other kinases, Pfmap1 and human MAPK1, suggesting that the Pfnek3-Pfmap2 interaction may be specific for Pfmap2 regulation. In summary, our data reveal a malarial NIMA-kinase with the potential to regulate a MAPK. Possessing biochemical properties divergent from classical mammalian NIMA-kinases, Pfnek3 could potentially be an attractive target for parasite-selective anti-malarials. © 2006 Federation of European Biochemical Societies.
Source Title: FEBS Letters
ISSN: 00145793
DOI: 10.1016/j.febslet.2006.10.003
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.