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https://scholarbank.nus.edu.sg/handle/10635/246261
DC Field | Value | |
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dc.title | ROLE OF RAB11 IN ENTEROVIRUS 71 INFECTION CYCLE | |
dc.contributor.author | NG QING YONG | |
dc.date.accessioned | 2023-11-30T18:00:40Z | |
dc.date.available | 2023-11-30T18:00:40Z | |
dc.date.issued | 2023-05-25 | |
dc.identifier.citation | NG QING YONG (2023-05-25). ROLE OF RAB11 IN ENTEROVIRUS 71 INFECTION CYCLE. ScholarBank@NUS Repository. | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/246261 | |
dc.description.abstract | Enterovirus A71 (EV-A71) is a major causative agent of Hand, Foot and Mouth Disease (HFMD) and is associated with severe neurological complications. A siRNA screen using murine motor neuron-like NSC34 cells, identified Rab11a as a pro-viral host factor during EV-A71 infection. While validation using deconvoluted siRNA confirmed Rab11a's pro-viral nature in NSC34 cells, co-knockdown of Rab11a and Rab11b isoforms in neuroblastoma SH-SY5Y and rhabdomyosarcoma RD cell lines was required to impact EV-A71 infection, suggesting interchangeability of both isoforms. Rab11's role in infection is highly conserved across enteroviruses and different EV-A71 subgenotypes and CVA16. I demonstrated that Rab11 does not play a role in viral RNA synthesis, viral entry/uncoating, or IRES-dependent translation. Also, unlike previous studies, I found that Rab11’s vesicle trafficking function is dispensable during EV-A71 infection. Instead, my work supports that Rab11 acts as an adapter protein at the replication organelles, facilitating EV-A71 assembly/maturation by recruiting chaperones/chaperonins. | |
dc.language.iso | en | |
dc.subject | Enterovirus A71 (EV-A71), Hand Foot and Mouth Disease (HFMD), Rab11, replication complex, CCT8, chaperonin-containing T-complex (TRiC) | |
dc.type | Thesis | |
dc.contributor.department | DEAN'S OFFICE (MEDICINE) | |
dc.contributor.supervisor | Sylvie Alonso | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY (SOM) | |
dc.identifier.orcid | 0009-0006-9071-8260 | |
Appears in Collections: | Ph.D Theses (Open) |
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