Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2009.01.101
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dc.titleComprehensive high-performance liquid chromatographic method for the measurements of lipophilic antioxidants in human plasma
dc.contributor.authorLee, B.L.
dc.contributor.authorOng, C.N.
dc.date.accessioned2011-07-26T02:52:05Z
dc.date.available2011-07-26T02:52:05Z
dc.date.issued2009
dc.identifier.citationLee, B.L., Ong, C.N. (2009). Comprehensive high-performance liquid chromatographic method for the measurements of lipophilic antioxidants in human plasma. Journal of Chromatography A 1216 (15) : 3131-3137. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2009.01.101
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24572
dc.description.abstractOwing to the increasing interest in the health effects of antioxidant micronutrients on chronic diseases, a robust and rapid HPLC method for simultaneous measurement of coenzyme Q10 (ubiquinone and ubiquinol), vitamin A (all-trans-retinol), vitamin E (tocopherols and tocotrienols) and carotenoids (lutein, zeaxanthin, β-cryptoxanthin, lycopene and β-carotene) was developed. Sample preparation and analytical conditions that would affect solubility and stability of these antioxidants were investigated and optimized. The mobile phase used was made up of acetonitrile, methanol, ethanol and tert-butanol without corrosive additives such as ammonium perchlorate and perchloric acid. Our results show that using two C18 columns coupled with photodiode array, fluorescence and electrochemical detection, a comprehensive spectrum of 16 lipid-soluble antioxidants in 30 μL of plasma could be separated and quantified within 30 min. The chromatographic run time was about 3-fold faster and the sample size was about 5-fold smaller than when assays were performed separately using existing methods. The present method will be useful for dietary habit studies and for antioxidant status investigations. © 2009 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.chroma.2009.01.101
dc.sourceScopus
dc.subjectAntioxidants
dc.subjectCoenzyme Q10
dc.subjectColumn-switching
dc.subjectFat-soluble
dc.subjectVitamins
dc.typeArticle
dc.contributor.departmentEPIDEMIOLOGY & PUBLIC HEALTH
dc.description.doi10.1016/j.chroma.2009.01.101
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume1216
dc.description.issue15
dc.description.page3131-3137
dc.identifier.isiut000265159700022
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