Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bbrc.2008.07.078
DC Field | Value | |
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dc.title | AFM indentation study of breast cancer cells | |
dc.contributor.author | Li, Q.S. | |
dc.contributor.author | Lim, C.T. | |
dc.contributor.author | Lee, G.Y.H. | |
dc.contributor.author | Ong, C.N. | |
dc.date.accessioned | 2011-07-25T06:58:58Z | |
dc.date.available | 2011-07-25T06:58:58Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Li, Q.S., Lim, C.T., Lee, G.Y.H., Ong, C.N. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications 374 (4) : 609-613. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.07.078 | |
dc.identifier.issn | 0006291X | |
dc.identifier.issn | 10902104 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/24463 | |
dc.description.abstract | Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM imaging and confocal fluorescence imaging were also used to investigate their corresponding sub-membrane cytoskeletal structures. Malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 °C), and their apparent Young's modulus increase with loading rate. Both confocal and AFM images showed a significant difference in the organization of their sub-membrane actin structures which directly contribute to their difference in cell elasticity. This change may have facilitated easy migration and invasion of malignant cells during metastasis. © 2008 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2008.07.078 | |
dc.source | Scopus | |
dc.subject | Actin cytoskeleton | |
dc.subject | Atomic force microscopy | |
dc.subject | Breast cancer disease | |
dc.subject | Cell mechanics | |
dc.subject | Confocal microscopy | |
dc.subject | Elasticity | |
dc.subject | Hertz's contact model | |
dc.subject | Stress fiber | |
dc.type | Article | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.description.doi | 10.1016/j.bbrc.2008.07.078 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 374 | |
dc.description.issue | 4 | |
dc.description.page | 609-613 | |
dc.identifier.isiut | 000258866900003 | |
Appears in Collections: | Staff Publications |
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