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|Title:||AFM indentation study of breast cancer cells||Authors:||Li, Q.S.
Atomic force microscopy
Breast cancer disease
Hertz's contact model
|Issue Date:||2008||Citation:||Li, Q.S., Lim, C.T., Lee, G.Y.H., Ong, C.N. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications 374 (4) : 609-613. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.07.078||Abstract:||Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM imaging and confocal fluorescence imaging were also used to investigate their corresponding sub-membrane cytoskeletal structures. Malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 °C), and their apparent Young's modulus increase with loading rate. Both confocal and AFM images showed a significant difference in the organization of their sub-membrane actin structures which directly contribute to their difference in cell elasticity. This change may have facilitated easy migration and invasion of malignant cells during metastasis. © 2008 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/24463||ISSN:||0006291X
|Appears in Collections:||Staff Publications|
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