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https://doi.org/10.1016/j.bbrc.2008.07.078
Title: | AFM indentation study of breast cancer cells | Authors: | Li, Q.S. Lim, C.T. Lee, G.Y.H. Ong, C.N. |
Keywords: | Actin cytoskeleton Atomic force microscopy Breast cancer disease Cell mechanics Confocal microscopy Elasticity Hertz's contact model Stress fiber |
Issue Date: | 2008 | Citation: | Li, Q.S., Lim, C.T., Lee, G.Y.H., Ong, C.N. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications 374 (4) : 609-613. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.07.078 | Abstract: | Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM imaging and confocal fluorescence imaging were also used to investigate their corresponding sub-membrane cytoskeletal structures. Malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 °C), and their apparent Young's modulus increase with loading rate. Both confocal and AFM images showed a significant difference in the organization of their sub-membrane actin structures which directly contribute to their difference in cell elasticity. This change may have facilitated easy migration and invasion of malignant cells during metastasis. © 2008 Elsevier Inc. All rights reserved. | Source Title: | Biochemical and Biophysical Research Communications | URI: | http://scholarbank.nus.edu.sg/handle/10635/24463 | ISSN: | 0006291X 10902104 |
DOI: | 10.1016/j.bbrc.2008.07.078 |
Appears in Collections: | Staff Publications |
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