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|Title:||Critical role of pro-apoptotic Bcl-2 family members in andrographolide-induced apoptosis in human cancer cells||Authors:||Zhou, J.
|Issue Date:||2006||Citation:||Zhou, J., Zhang, S., Choon-Nam, O., Shen, H.-M. (2006). Critical role of pro-apoptotic Bcl-2 family members in andrographolide-induced apoptosis in human cancer cells. Biochemical Pharmacology 72 (2) : 132-144. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bcp.2006.04.019||Abstract:||Andrographolide (Andro), a diterpenoid lactone isolated from a traditional herbal medicine Andrographis paniculata, is known to possess potent anti-inflammatory activity. In this study, Andro induced apoptosis in human cancer cells via activation of caspase 8 in the extrinsic death receptor pathway and subsequently with the participation of mitochondria. Andro triggered a caspase 8-dependent Bid cleavage, followed by a series of sequential events including Bax conformational change and mitochondrial translocation, cytochrome c release from mitochondria, and activation of caspase 9 and 3. Inhibition of caspase 8 blocked Bid cleavage and Bax conformational change. Consistently, knockdown of Bid protein using small interfering RNA (siRNA) technique suppressed Andro-induced Bax conformational change and apoptosis. In conclusion, the pro-apoptotic Bcl-2 family members (Bid and Bax) are the key mediators in relaying the cell death signaling initiated by Andro from caspase 8 to mitochondria and then to downstream effector caspases, and eventually leading to apoptotic cell death. © 2006 Elsevier Inc. All rights reserved.||Source Title:||Biochemical Pharmacology||URI:||http://scholarbank.nus.edu.sg/handle/10635/24438||ISSN:||00062952||DOI:||10.1016/j.bcp.2006.04.019|
|Appears in Collections:||Staff Publications|
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