Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aca.2008.01.045
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dc.titleSimultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction
dc.contributor.authorMa, Z.
dc.contributor.authorGe, L.
dc.contributor.authorLee, A.S.Y.
dc.contributor.authorYong, J.W.H.
dc.contributor.authorTan, S.N.
dc.contributor.authorOng, E.S.
dc.date.accessioned2011-07-25T06:58:33Z
dc.date.available2011-07-25T06:58:33Z
dc.date.issued2008
dc.identifier.citationMa, Z., Ge, L., Lee, A.S.Y., Yong, J.W.H., Tan, S.N., Ong, E.S. (2008). Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta 610 (2) : 274-281. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aca.2008.01.045
dc.identifier.issn00032670
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24437
dc.description.abstractCoconut (Cocos nucifera L.) water, which contains many uncharacterized phytohormones is extensively used as a growth promoting supplement in plant tissue culture. In this paper, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of various classes phytohormones, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in young coconut water (CW). The analysis was carried out using a reverse-phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid, pH adjusted to 3.2 with triethylamine (TEA)) modified by methanol, and solute detection made at 265 nm wavelength. The method was validated for specificity, quantification, accuracy and precision. After preconcentration of putative endogenous phytohormones in CW using C18 solid-phase extraction (SPE) cartridges, the HPLC method was able to screen for putative endogenous phytohormones present in CW. Finally, the identities of the putative phytohormones present in CW were further confirmed using independent liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an electrospray ionization (ESI) interface. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.aca.2008.01.045
dc.sourceScopus
dc.subjectCoconut water
dc.subjectHigh-performance liquid chromatography
dc.subjectLiquid chromatography-tandem mass spectrometry
dc.subjectPhytohormones
dc.typeArticle
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1016/j.aca.2008.01.045
dc.description.sourcetitleAnalytica Chimica Acta
dc.description.volume610
dc.description.issue2
dc.description.page274-281
dc.identifier.isiut000254101800016
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