Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neulet.2009.03.038
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dc.titleApolipoprotein D modulates F2-isoprostane and 7-ketocholesterol formation and has a neuroprotective effect on organotypic hippocampal cultures after kainate-induced excitotoxic injury
dc.contributor.authorHe, X.
dc.contributor.authorJittiwat, J.
dc.contributor.authorKim, J.-H.
dc.contributor.authorOng, W.-Y.
dc.contributor.authorJenner, A.M.
dc.contributor.authorFarooqui, A.A.
dc.contributor.authorPatel, S.C.
dc.date.accessioned2011-07-25T02:43:36Z
dc.date.available2011-07-25T02:43:36Z
dc.date.issued2009
dc.identifier.citationHe, X., Jittiwat, J., Kim, J.-H., Ong, W.-Y., Jenner, A.M., Farooqui, A.A., Patel, S.C. (2009). Apolipoprotein D modulates F2-isoprostane and 7-ketocholesterol formation and has a neuroprotective effect on organotypic hippocampal cultures after kainate-induced excitotoxic injury. Neuroscience Letters 455 (3) : 183-186. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neulet.2009.03.038
dc.identifier.issn03043940
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24366
dc.description.abstractApolipoprotein D (apoD), a member of the lipocalin family of transporter proteins binds a number of small lipophilic molecules including arachidonic acid and cholesterol. Recent studies showed a protective function of mammalian apoD as well as its insect and plant homologs against oxidative stress. In this study we investigated the effect of direct addition of exogenous human apoD protein purified from breast cystic fluid to rat hippocampal slice cultures after excitotoxic injury induced by the glutamate analog kainate. ApoD at a concentration of 10 μg/ml partially prevented loss of MAP2 immunostaining and LDH release from injured hippocampal neurons after kainate injury. ApoD also attenuated the increase in oxidative products of arachidonic acid and cholesterol, F2-isoprostanes and 7-ketocholesterol, respectively, after kainate treatment. In view of the molecular structure of apoD which consists of an eight stranded β barrel that forms a binding pocket for a number of small hydrophobic molecules, we propose that apoD promotes its neuroprotective effects by binding to arachidonic acid and cholesterol thus preventing their oxidation to neurotoxic products such as 4-hydroxynonenal (4-HNE) and 7-ketocholesterol. © 2009 Elsevier Ireland Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.neulet.2009.03.038
dc.sourceScopus
dc.subject7-Ketocholesterol
dc.subjectApolipoprotein D
dc.subjectExcitotoxicity
dc.subjectLipid peroxidation
dc.subjectOxidative stress
dc.subjectOxysterols
dc.typeArticle
dc.contributor.departmentANATOMY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.neulet.2009.03.038
dc.description.sourcetitleNeuroscience Letters
dc.description.volume455
dc.description.issue3
dc.description.page183-186
dc.identifier.isiut000265725800007
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