Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cbpc.2008.06.003
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dc.titleIsolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi
dc.contributor.authorGao, R.
dc.contributor.authorGopalakrishnakone, P.
dc.date.accessioned2011-07-25T02:42:44Z
dc.date.available2011-07-25T02:42:44Z
dc.date.issued2008
dc.identifier.citationGao, R., Gopalakrishnakone, P. (2008). Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi. Comparative Biochemistry and Physiology - C Toxicology and Pharmacology 148 (3) : 250-257. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cbpc.2008.06.003
dc.identifier.issn15320456
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24314
dc.description.abstractA hyaluronidase, named BmHYA1, was purified from the venom of Chinese red scorpion (Buthus martensi), using successive chromatography. The homogeneity of BmHYA1 was confirmed by SDS-PAGE and MALDI-TOF mass spectrometry. The molecular mass of BmHYA1 was 48,696 Da determined by MALDI-TOF MS. The optimal temperature and pH of BmHYA1 were 50 °C and pH 4.5, respectively. It could be inhibited by DTT, Cu2+, Fe3+ or heparin, but not Mg2+, Ca2+, reduced glutathione, l-cysteine or EDTA. The sequence of thirty N-terminal amino acids of BmHYA1 was obtained by Edman degradation, as TSADF KVVWE VPSIM CSKKF KICVT DLLTS; but no similarity was found to other venom hyaluronidases. Further, BmHYA1 can hydrolyze hyaluronan into relatively smaller oligosaccharides and modulate the expression of CD44 variant in the breast cancer cell line MDA-MB-231. © 2008 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cbpc.2008.06.003
dc.sourceScopus
dc.subjectCD44
dc.subjectHyaluronidase
dc.subjectN-terminal sequence
dc.subjectPurification
dc.subjectScorpion venom
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentANATOMY
dc.description.doi10.1016/j.cbpc.2008.06.003
dc.description.sourcetitleComparative Biochemistry and Physiology - C Toxicology and Pharmacology
dc.description.volume148
dc.description.issue3
dc.description.page250-257
dc.identifier.isiut000259350900009
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