Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scr.2009.12.003
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dc.titleExosome secreted by MSC reduces myocardial ischemia/reperfusion injury
dc.contributor.authorLai, R.C.
dc.contributor.authorChen, T.S.
dc.contributor.authorLim, S.K.
dc.contributor.authorArslan, F.
dc.contributor.authorTimmers, L.
dc.contributor.authorPasterkamp, G.
dc.contributor.authorde, Kleijn D.P.V.
dc.contributor.authorLee, M.M.
dc.contributor.authorCHOO BOON HWA,ANDRE
dc.contributor.authorSze, N.S.K.
dc.contributor.authorChoo, A.
dc.contributor.authorSalto-Tellez, M.
dc.contributor.authorLee, C.N.
dc.contributor.authorEl, Oakley R.M.
dc.date.accessioned2011-07-19T10:13:38Z
dc.date.available2011-07-19T10:13:38Z
dc.date.issued2010
dc.identifier.citationLai, R.C., Chen, T.S., Lim, S.K., Arslan, F., Timmers, L., Pasterkamp, G., de, Kleijn D.P.V., Lee, M.M., CHOO BOON HWA,ANDRE, Sze, N.S.K., Choo, A., Salto-Tellez, M., Lee, C.N., El, Oakley R.M. (2010). Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Research 4 (3) : 214-222. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2009.12.003
dc.identifier.issn18735061
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24200
dc.description.abstractHuman ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50-100 nm. Here we show that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous population of particles with a hydrodynamic radius of 55-65 nm by size-exclusion fractionation on a HPLC. Together these observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes. This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury and repair, and engenders novel approaches to the development of biologics for tissue repair. © 2009 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.scr.2009.12.003
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentDIVISION OF BIOENGINEERING
dc.contributor.departmentPATHOLOGY
dc.contributor.departmentSURGERY
dc.description.doi10.1016/j.scr.2009.12.003
dc.description.sourcetitleStem Cell Research
dc.description.volume4
dc.description.issue3
dc.description.page214-222
dc.identifier.isiut000279233100007
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