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Title: Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury
Authors: Lai, R.C.
Chen, T.S. 
Lim, S.K. 
Arslan, F.
Timmers, L.
Pasterkamp, G.
de, Kleijn D.P.V.
Lee, M.M.
Sze, N.S.K.
Choo, A. 
Salto-Tellez, M. 
Lee, C.N. 
El, Oakley R.M. 
Issue Date: 2010
Citation: Lai, R.C., Chen, T.S., Lim, S.K., Arslan, F., Timmers, L., Pasterkamp, G., de, Kleijn D.P.V., Lee, M.M., CHOO BOON HWA,ANDRE, Sze, N.S.K., Choo, A., Salto-Tellez, M., Lee, C.N., El, Oakley R.M. (2010). Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Research 4 (3) : 214-222. ScholarBank@NUS Repository.
Abstract: Human ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50-100 nm. Here we show that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous population of particles with a hydrodynamic radius of 55-65 nm by size-exclusion fractionation on a HPLC. Together these observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes. This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury and repair, and engenders novel approaches to the development of biologics for tissue repair. © 2009 Elsevier B.V. All rights reserved.
Source Title: Stem Cell Research
ISSN: 18735061
DOI: 10.1016/j.scr.2009.12.003
Appears in Collections:Staff Publications

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