Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jtcvs.2006.08.001
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dc.titleExpression of neuronal nitric oxide synthase in the internal thoracic artery and saphenous vein
dc.contributor.authorWebb, G.D.
dc.contributor.authorLim, L.H.
dc.contributor.authorMoore, P.K.
dc.contributor.authorOh, V.M.S.
dc.contributor.authorEl, Oakley R.
dc.contributor.authorLee, C.N.
dc.contributor.authorWong, P.S.
dc.contributor.authorAye, W.M.M.
dc.contributor.authorChan, E.S.Y.
dc.date.accessioned2011-07-19T10:13:33Z
dc.date.available2011-07-19T10:13:33Z
dc.date.issued2006
dc.identifier.citationWebb, G.D., Lim, L.H., Moore, P.K., Oh, V.M.S., El, Oakley R., Lee, C.N., Wong, P.S., Aye, W.M.M., Chan, E.S.Y. (2006). Expression of neuronal nitric oxide synthase in the internal thoracic artery and saphenous vein. Journal of Thoracic and Cardiovascular Surgery 132 (5) : 1131-1136. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jtcvs.2006.08.001
dc.identifier.issn00225223
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24195
dc.description.abstractObjectives: Endothelial nitric oxide synthase (type III) generates nitric oxide, which dilates blood vessels. Recently, it was discovered that arterial smooth muscle cells express neuronal nitric oxide synthase (type I). The purpose of this study was to determine the relative amounts of neuronal nitric oxide synthase in the human internal thoracic artery and saphenous vein. Methods: Remainder segments of internal thoracic arteries and saphenous veins were obtained from 45 patients during coronary artery bypass grafting. Western blotting used specific antibodies against the 3 isoforms of human nitric oxide synthase and β-actin (for normalization) to measure the relative amounts of the 3 isoforms of nitric oxide synthase proteins in vessel specimens. Immunohistochemistry was used to localize the 3 proteins in specific cells. Results: Western blotting detected all 3 isoforms of nitric oxide synthase in the human internal thoracic artery. The band density (normalized to β-actin) of neuronal nitric oxide synthase was not significantly different from the band density of endothelial nitric oxide synthase. The amounts of neuronal nitric oxide synthase in arteries and veins were equal. Immunohistochemistry showed that the highest expression of endothelial nitric oxide synthase was in endothelial cells, but some expression was also seen in smooth muscle cells. Most of the neuronal nitric oxide synthase was in smooth muscle cells. The location and relative amounts of inducible nitric oxide synthase were variable. Conclusions: Neuronal nitric oxide synthase is expressed in the vascular smooth muscle of patients undergoing bypass, and the amount in the internal thoracic artery is the same as in the saphenous vein. © 2006 The American Association for Thoracic Surgery.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jtcvs.2006.08.001
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentPHARMACOLOGY
dc.contributor.departmentMEDICINE
dc.description.doi10.1016/j.jtcvs.2006.08.001
dc.description.sourcetitleJournal of Thoracic and Cardiovascular Surgery
dc.description.volume132
dc.description.issue5
dc.description.page1131-1136
dc.identifier.isiut000241497500020
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