Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.01.014
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dc.titleAngiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repair
dc.contributor.authorYe, L.
dc.contributor.authorHaider, H.Kh.
dc.contributor.authorTan, R.
dc.contributor.authorSu, L.
dc.contributor.authorZhang, W.
dc.contributor.authorLaw, P.K.
dc.contributor.authorSim, E.K.W.
dc.date.accessioned2011-07-19T10:13:24Z
dc.date.available2011-07-19T10:13:24Z
dc.date.issued2008
dc.identifier.citationYe, L., Haider, H.Kh., Tan, R., Su, L., Zhang, W., Law, P.K., Sim, E.K.W. (2008). Angiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repair. Biomaterials 29 (13) : 2125-2137. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.01.014
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24186
dc.description.abstractWe aim to investigate the feasibility and efficacy of cholesterol (Chol) + DOTAP liposome (CD liposome) based human vascular endothelial growth factor-165 (hVEGF165) gene transfer into human skeletal myoblasts (hSkM) for cardiac repair. The feasibility and efficacy of CD liposome for gene transfer with hSkM was characterized using plasmid carrying enhanced green fluorescent protein (pEGFP). Based on the optimized transfection procedure, hSkM were transfected with CD lipoplexes carrying plasmid-hVEGF165 (CD-phVEGF165). The genetically modified hSkM were transplanted into rat heart model of acute myocardial infarction. Flow cytometry revealed that about 7.99% hSkM could be transfected with pEGFP. Based on the optimized transfection condition, transfected hSkM expressed hVEGF165 up to day-18 (1.7 ± 0.1 ng/ml) with peak at day-2 (13.1 ± 0.52 ng/ml) with >85% cell viability. Animal studies revealed that reduced apoptosis, improved angiogenesis with blood flow in group-3 animal's heart were achieved as compared to group-1 and 2. Ejection fraction was best recovered in group-3 animals. The study demonstrates that though gene transfection efficiency using CD liposome mediated hVEGF165 gene transfer with hSkM was low; hVEGF165 gene expression efficiency was sufficient to induce neovascularization, improve blood flow and injured heart function. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.01.014
dc.sourceScopus
dc.subjectCardiac repair
dc.subjectLiposome
dc.subjectSkeletal myoblast
dc.subjectVEGF165
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1016/j.biomaterials.2008.01.014
dc.description.sourcetitleBiomaterials
dc.description.volume29
dc.description.issue13
dc.description.page2125-2137
dc.identifier.isiut000256853100013
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