Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.canlet.2008.10.038
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dc.titleSilencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells
dc.contributor.authorLim, D.
dc.contributor.authorJocelyn, K.M.-X.
dc.contributor.authorYip, G.W.-C.
dc.contributor.authorBay, B.-H.
dc.date.accessioned2011-07-18T08:44:28Z
dc.date.available2011-07-18T08:44:28Z
dc.date.issued2009
dc.identifier.citationLim, D., Jocelyn, K.M.-X., Yip, G.W.-C., Bay, B.-H. (2009). Silencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells. Cancer Letters 276 (1) : 109-117. ScholarBank@NUS Repository. https://doi.org/10.1016/j.canlet.2008.10.038
dc.identifier.issn03043835
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/23911
dc.description.abstractMetallothioneins (MTs) are a group of metal-binding proteins involved in cell proliferation, differentiation and apoptosis. The MT-2A isoform is generally the most abundant isoform among the 10 known functional MT genes. In the present study, we observed that down-regulation of the MT-2A gene in MCF-7 cells via siRNA-mediated silencing inhibited cell growth by inducing cell cycle arrest in G1-phase (G1-arrest) and a marginal increase in cells in sub-G1-phase. Scanning electron microscopic examination of the cells with silenced expression of MT-2A (siMT-2A cells) revealed essentially normal cell morphology with presence of scattered apoptotic cells. To elucidate the underlying molecular mechanism, we examined the expression of cell cycle related genes in MT-2A-silenced cells and found a higher expression of the ataxia telangiectasia mutated (ATM) gene concomitant with a lower expression of the cdc25A gene. These data suggest that MT-2A could plausibly modulate cell cycle progression from G1- to S-phase via the ATM/Chk2/cdc25A pathway. © 2008 Elsevier Ireland Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.canlet.2008.10.038
dc.sourceScopus
dc.subjectATM
dc.subjectBreast cancer
dc.subjectcdc25A
dc.subjectCell cycle
dc.subjectMetallothionein
dc.typeArticle
dc.contributor.departmentANATOMY
dc.description.doi10.1016/j.canlet.2008.10.038
dc.description.sourcetitleCancer Letters
dc.description.volume276
dc.description.issue1
dc.description.page109-117
dc.identifier.isiut000264581900016
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