Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms24021259
Title: Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases
Authors: Go, Maybelle Kho 
Zhu, Tingting 
Lim, Kevin Jie Han 
Hartono, Yossa Dwi 
Xue, Bo 
Fan, Hao 
Yew, Wen Shan 
Keywords: cannabinoid biosynthesis
cannabinoid synthase
berberine bridge enzyme
cannabielsoin
cannabigerolic acid
Pichia pastoris
Issue Date: 9-Jan-2023
Publisher: MDPI
Citation: Go, Maybelle Kho, Zhu, Tingting, Lim, Kevin Jie Han, Hartono, Yossa Dwi, Xue, Bo, Fan, Hao, Yew, Wen Shan (2023-01-09). Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (2). ScholarBank@NUS Repository. https://doi.org/10.3390/ijms24021259
Abstract: We report enzymes from the berberine bridge enzyme (BBE) superfamily that catalyze the oxidative cyclization of the monoterpene moiety in cannabigerolic acid (CBGA) to form cannabielsoin (CBE). The enzymes are from a variety of organisms and are previously uncharacterized. Out of 232 homologues chosen from the enzyme superfamily, four orthologues were shown to accept CBGA as a substrate and catalyze the biosynthesis of CBE. The four enzymes discovered in this study were recombinantly expressed and purified in Pichia pastoris. These enzymes are the first report of heterologous expression of BBEs that did not originate from the Cannabis plant that catalyze the production of cannabinoids using CBGA as substrate. This study details a new avenue for discovering and producing natural and unnatural cannabinoids.
Source Title: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
URI: https://scholarbank.nus.edu.sg/handle/10635/238292
ISSN: 1661-6596
1422-0067
DOI: 10.3390/ijms24021259
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